Seaweed flavoured tuna bone gelatine edible film and preparation method thereof
A technology of fish bone gelatin and tuna, applied in the direction of sustainable manufacturing/processing, food science, climate sustainability, etc., to achieve the effect of increasing added value, extending shelf life, and increasing antioxidant effect
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[0026] (1) Pretreatment of tuna fish bones: Soak in 0.1 mol / L NaOH solution at 4°C for 5 h according to the ratio of solid to liquid: 1 g:20 mL, to remove non-collagen; after the treatment, the fish bones were washed repeatedly with distilled water until pH 6.5 After fully draining, add 0.5 mol / L EDTA solution (pH7.4) according to the ratio of solid to liquid 1 g: 10 mL, soak at 4 °C for 4 days, replace the EDTA solution once a day, and remove the calcium in the fish bones. Dried and crushed to obtain decalcified fish bone meal.
[0027] (2) Preparation of tuna bone gelatin: add 4% HCl solution according to the ratio of solid to liquid 1 g: 8 mL, after 18 h of treatment, wash the fish bone powder with water to pH 7.0, and distilled water according to the ratio of solid to liquid 1 g: 10 mL Add it to fish bone powder, extract at 70°C for 8 h, filter, concentrate in vacuo, and dry in vacuo at 65°C to obtain fish bone gelatin.
[0028] (3) Preparation of seaweed extract: fresh E...
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