Platelet-rich plasma separation gel and platelet-rich plasma preparation method

Abstract

The invention discloses a platelet-rich plasma separation gel, a vacuum blood collection tube which is prepared by utilizing the separation gel and used for separating platelet-rich plasma, and a separation method. The platelet-rich plasma separation gel particularly comprises a resin, and the resin is prepared from the following raw materials in parts by weight: 120 parts of butyl acetate, 75-80 parts of phenethylene, 20-25 parts of butyl acrylate, 5-12 parts of acrylic acid, 2-2.5 parts of azodiisobutyronitrile (AIBN), and 0.5-0.8 parts of dodecyl mercaptan, and the specific weight ranges from 1.068 to 1.080 at the temperature of 25 DEG C and can be accurately adjusted according to the raw material ratio. The separation gel prepared by use of the resin and the manufactured vacuum blood collection tube are also included. When the blood collection tube is used for preparing platelet-rich plasma, the process is simple, convenient and easy, and the required time is short; repeated tube transfer is not required, so that cross contamination is avoided; and the operation is simple and convenient, the preparation cost is greatly reduced, the practicability and the feasibility are greatly improved, and a wide market prospect is achieved.

Description

technical field [0001] The invention belongs to the field of blood sampling and separation, and in particular relates to a platelet-rich plasma separation gel and a method for separating and preparing platelet-rich plasma by using the separation gel. Background technique [0002] Platelet-rich plasma (Platelet-rich plasma, PRP) is platelet-concentrated plasma obtained from autologous whole blood after centrifugation. Platelets contain a large number of growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), insulin-like growth factor (IGF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF) These growth factors are mainly secreted by platelet activation, which can promote the regeneration of cells and matrix in tissues from different aspects, thereby promoting tissue repair. [0003] Among the existing PRP preparation methods, according to the number of centrifugation, it can be divided into one-time, two-ti...

AI Technical Summary

Problems solved by technology

[0011] (1) After centrifugation and stratification, no effective isolation can be formed. Since the specific gravity of platelets is close to that of blood cells, most of the platelets will stop at the junction of plasma and blood cells. During the absorption process, it is easy to inhale blood cells, and it is easy to re-mix, resulting in failure to collect PRP;

[0012] (2) The open preparation system is susceptible to external contamination;

[0014] (4) The operator's personal habits and scales have greatly affected the concentration of platelets in PRP, resulting in a low recovery rate of platelets in the prepared PRP

[0015] Therefore, the enrichment coefficient of the prepared platelet-rich plasma is low, the platelet recovery is incomplete, the recovery rate is low, the operation is cumbersome, and human errors or even mistakes are prone to occur, resulting in a small amount of PRP or even invalid PRP.

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