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Method for extracting aureusidin from eleocharis tuberosa peels

A technology of snapdragon and water chestnut skin, applied in the direction of organic chemistry and the like, can solve unseen and other problems, achieve the effects of low production cost, high product purity, and meet the needs of the pharmaceutical industry

Active Publication Date: 2015-04-29
贺州市顺来农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In our research, we found that water chestnut skin is rich in aureusidin, but there is no literature report on the extraction and isolation of this compound from water chestnut skin

Method used

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  • Method for extracting aureusidin from eleocharis tuberosa peels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In the extraction tank, add 800g of fresh water chestnut skin powder and 6.4L of 50% acetone aqueous solution, extract at 50℃ for 2h, extract 3 times in total, filter, combine the filtrate, concentrate under reduced pressure into a paste to obtain 120g of extract .

[0022] 900 mL of water was added to the extract to make a turbid liquid, and 1.8L of ethyl acetate was added to extract 3 times, the extracts were combined and concentrated under reduced pressure to obtain 10.5 g of extract.

[0023] Dissolve the extract in 25mL methanol, add 250mL water to make a suspension, transfer it to a 1kg D101 macroporous resin chromatography column for adsorption, after static adsorption, wash with water until the effluent is colorless, and then use 25% by volume 800mL methanol aqueous solution to wash away the polar glycoside impurities, and finally eluted with 50% methanol aqueous solution by volume percentage, detected by thin-layer chromatography, collected and combined the eluent c...

Embodiment 2

[0026] In the extraction tank, add 800g of fresh water chestnut peel powder and 1.6L of 75% methanol aqueous solution, extract at 70℃ for 2.5h, filter, extract 3 times in total, combine the filtrate and concentrate the paste under reduced pressure to obtain 150g of extract .

[0027] 750 mL of water was added to the extract to make a turbid liquid, then 1.5L of ethyl acetate was added to extract 3 times, the extracts were combined, and concentrated under reduced pressure to obtain 12.0 g of extract.

[0028] Dissolve the extract in 24mL of methanol, then add 240mL of water to make a suspension, transfer it to a 1kg S-8 macroporous resin chromatography column for adsorption, after static adsorption, wash with water until the effluent is colorless, and then use 25 800mL of methanol aqueous solution of% volume percentage to wash away the polar glycoside impurities, and finally eluted with 50% volume percentage of methanol aqueous solution, detection by thin layer chromatography, colle...

Embodiment 3

[0031] In the extraction tank, add 800g of fresh water chestnut skin powder and 8.0L of 70% ethanol aqueous solution, extract at 65℃ for 1.5h, extract 2 times, filter, combine the filtrate, concentrate under reduced pressure into a paste to obtain the extract 100g.

[0032] 800 mL of water was added to the extract to make a turbid liquid, 1.6L of ethyl acetate was added to extract 3 times, the extracts were combined, and concentrated under reduced pressure to obtain 9.5 g of extract.

[0033] Dissolve the extract in 19mL methanol, add 190mL water to make a suspension, transfer it to a 1kg D101 macroporous resin chromatography column for adsorption, after static adsorption, wash with water until the effluent is colorless, and then use 25% by volume 800mL of ethanol aqueous solution to wash away the large polar glycoside impurities, and finally eluted with 45% ethanol aqueous solution by volume percentage, thin-layer chromatography detection, collect and merge the eluate containing S...

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Abstract

The invention discloses a method for extracting aureusidin from eleocharis tuberosa peels, and belongs to the field of natural organic chemistry. The method is characterized by comprising the following steps: taking any one of methanol aqueous solution, ethanol aqueous solution and acetone aqueous solution as the solvent, extracting aureusidin from eleocharis tuberosa peels, separating the extract with macroporous adsorption resin after extraction with ethyl acetate, and purifying the obtained crude products with a gel chromatography column to obtain aureusidin products with the purity of above 95%. According to the method for extracting aureusidin from eleocharis tuberosa peels, a large number of waste eleocharis tuberosa peels are used as the raw materials for preparing aureusidin, so that the waste eleocharis tuberosa peels are recycled, and a new approach to extract and separate aureusidin from natural plants is provided; the separation effect is good; the purity of products is high; the selected macroporous resin and gel can be used repeatedly; the method has the advantages of accessible raw materials, abundant resources and low production cost, can perform large-scale production, and can meet requirements of the pharmaceutical industry.

Description

Technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting and separating snapdragon, in particular, to a method for extracting and separating snapdragon from water chestnut peel. Background technique [0002] Aureusidin, whose chemical name is 4,6,3',4'-tetrahydroxy orange ketone, is the most famous orange ketone compound and has multiple biological activities such as antioxidant, antibacterial, and antitumor. Antirrhinum has a special structure, is less distributed in natural plants, and has a low content in plants, which makes it difficult to obtain on a large scale. In order to facilitate the study of its pharmacological activity, scholars usually use synthetic methods to obtain antirrhinum. Hu Kun of Changzhou University reported on the total synthesis method of antirrhinum, which uses 2,4,6-trihydroxyacetophenone and 3,4-dihydroxybenzaldehyde as raw materials to synthesize antirrhinum through a five-step r...

Claims

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Application Information

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IPC IPC(8): C07D307/83
CPCC07D307/83
Inventor 罗杨合何星存李行任谢玉花黄永兰
Owner 贺州市顺来农业发展有限公司
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