Special strain culture medium for mushroom liquefaction and corresponding culture method

Abstract

The invention discloses a special strain culture medium for mushroom liquefaction. The special strain culture medium is prepared from the following components in parts by weight: 40-50 parts of corn starch, 1-2 parts of glucose, 15-20 parts of wheat cellulose, 15-23 parts of fine bran, 1-1. 5 parts of a yeast extract, 1-1.5 parts of beef peptone, 2-3 parts of potassium dihydrogen phosphate, 1-1.5 parts of magnesium sulfate, 2 parts of gypsum and 100 parts of water. The invention further discloses a strain culture method for carrying out mushroom liquefaction by employing the culture medium. The culture method sequentially comprises the following steps: carrying out high-temperature and high-pressure sterilization on a special strain culture medium for mushroom liquefaction; inoculating into a mushroom mother strain in the sterilized culture medium under a sterile condition, and carrying out darkness culture at 22-24 DEG C for 25-26 days; and carrying out dilution treatment on the obtained special solid strain for liquefaction so as to obtain the liquefied mushroom strain.

Description

technical field [0001] The invention relates to a formula and a cultivation method of bacteria special for liquefaction of shiitake mushrooms. Background technique [0002] Edible fungus is one of the advantageous industries with the most modern agricultural development characteristics in China. This is not only because China is the world's largest producer and consumer of edible fungi, with a wide range of edible fungus production, but more importantly, the edible fungus industry is a good carrier and prominent position in the industrialization of biotechnology and the sustainable development of agriculture. At present, my country's edible fungus industry system has many factors that are not suitable for the global market economy, the most important of which are the intensification of industrial operations, low degree of specialization, backward production factors, imperfect production technology, and lack of key technology support. The most representative one is the intens...

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Problems solved by technology

Remarks: The liquid fermentation strain process theoretically has a high purity of the strain, and the bacterial contamination rate of large-scale fermentation production is between 1% and 5% (in a 3-stage fermenter), and the bacterial contamination (mainly bacterial) is often In the later stage of fermentation, it is difficult to find out by conventional on-line inspection, which leads to production accidents caused by the inoculation of liquid fermentation strains and the contamination of large quantities of mushroom bags, which is a painful lesson.

In addition, because the cultivation of liquid strains requires nutrients such as high-concentration organic nitrogen and sugar sources, these ingredients remain in the bacterial liquid and are inserted into the fruiting bag. Since bacteria are inevitably brought in during operation, the residual nutrients It becomes a breeding ground for miscellaneous bacteria, which increases the risk of contamination after inoculation of the fruiting bag

This is the main problem that liquid fermentation strains cannot be applied to the production of edible fungi on a large scale

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