Jatropha extracting method and antifungal application
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An extraction method and technology of Jatropha curcas are applied in the extraction method and antifungal application field of Jatropha curcas, to achieve the effects of less tolerance and less side effects
Inactive Publication Date: 2015-04-29
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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Problems solved by technology
In recent years, scholars at home and abroad have done a lot of research on the use of Jatropha curcas, and found that it has anti-tumor, anti-virus and other effects, but there are various deficiencies in its extraction method, and there is no external product report for anti-fungal
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Embodiment 1
[0012] Take the whole plant of Jatropha curcas, dry, remove impurities, crush, sieve, place in an ultrasonic extraction tank, add 10 times the amount of methanol, extract three times, 30 minutes each time, combine the extracts, concentrate under reduced pressure, and apply silica gel to the concentrate The column was sequentially eluted with different concentrations of ethanol (25%, 50%, 75%, 95%), and the active ingredients of flavonoids were monitored by thin-layer chromatography. The eluents containing flavonoids were combined, concentrated under reduced pressure, concentrated liquid for use. Add stearic acid, glyceryl monostearate, triethanolamine, ethylparaben, salicylic acid and NaOH to the concentrated solution, put it in a homogenizer, grind, and stir well.
Embodiment 2
[0013] Example 2 The extract obtained in Example 1 was subjected to an in vitro antifungal experiment, and the results showed that the extract had an in vitro antifungal effect. The specific method is as follows:
[0014] 1. Liquid preparation:
[0015] The Jatropha extract of Example 1 was dissolved in 100% DMSO (dimethyl sulfoxide) in a sterilized distilled water machine, and then diluted with sterile distilled water to make 2 mg / ml doubling dilution to an appropriate concentration.
[0016] 2. Test tube agar drug-based method
[0017] Mix the above-mentioned medicinal liquid with the ratio of 1:9 to the mold culture medium to make a medium containing a series of drug concentrations and put them into test tubes, 5ml in each tube, and set aside for use. Dilute each bacterium to be tested with 10 5 The amount of CFU / ml spores was planted into each tube, and incubated at 30°C for 24h (Candida albicans) and 26°C for 7d (Candida tropicalis), and a solvent, medium blank ...
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Abstract
The invention discloses a jatropha extracting method and antifungal application. The extracting method comprises the following steps: ultrasonically extracting a jatropha medicinal material by taking 10 times volume of methanol as an extracting solvent for three times, wherein each time lasts for 30min, combining extracting liquid, decompressing and concentrating, making a concentrated solution undergo column chromatography on silica gel, eluting in a gradient manner with ethanol differing in concentration, monitoring flavonoid active ingredients by virtue of thin layer chromatography, combining eluent containing the flavonoid active ingredients, decompressing and concentrating, and reserving a concentrated solution for later use. The jatropha extract disclosed by the invention has a significant effect of improving external fungal infection and is applicable to the treatment of skin fungal infection; the extract can be prepared into various dosage forms as required; and used for treating the fungal infection, the jatropha extract has the advantages of being low in side effect, not easy to cause tolerance and the like.
Description
technical field : [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a method for extracting Jatropha curcas and its antifungal application. Background technique : [0002] Jatropha curcas L. is a deciduous shrub or small tree of Euphorbiaceae, also known as tung tree, green jade tree, tung tree, stinky tung tree, green tung tree, tung oil tree, fake peanut tree, etc. According to "Guangxi Chinese Herbal Medicine", it is slightly cold in nature, astringent in taste, and poisonous. It can be used to treat swelling and pain from bruises, fractures, wounds, itchy skin, eczema, and acute gastroenteritis. Jatropha curcas is native to the Americas, and is now widely distributed in tropical, subtropical and dry hot valley regions. In my country, it is mainly distributed in Guangdong, Hainan, Fujian, Taiwan, Guangxi, Yunnan, Guizhou, Sichuan and other places. In recent years, scholars at home and abroad have done a lot of resea...
Claims
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Application Information
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