Method for producing feed through pretreating at high temperature and fermenting at low temperature
A low-temperature fermentation and pretreatment technology, applied in animal feed, animal feed, applications, etc., can solve the problems of not being able to meet the feeding needs of various animals, not being able to greatly improve the nutrition of feed raw materials, and the harm of chemicals to animals, so as to promote Adjustment of agricultural industry structure, solving the problem of feed protein shortage, alleviating the effects of humans and animals competing for food
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Embodiment 1
[0034] Slant medium: 1.0% glucose, 1.0% corn steep liquor, 2.0% agar, pH 7.5, sterilized at 115°C for 20 min.
[0035] Liquid medium: 1.0% corn bran, 1.0% corn steep liquor, CaCO 3 0.3%, filter paper 0.1%, pH 7.5, no sterilization required.
[0036] The following microorganisms: Bacillus coagulans, Bacillus licheniformis, Bacillus thermophilia, Proteus, chelating coccus, and lignocellulose-degrading bacteria were respectively inserted into the slant medium, cultured at 55°C for 3 days, and the strain slant was obtained; then six Each of the microorganisms was put into the liquid culture medium one by one, and cultured at 55° C. for 3 days to obtain the microorganism culture solution.
[0037] Mix corn husks, germ meal, corn steep liquor, and protein powder in amounts of 200 g, 180 g, 100 g (dry matter), and 110 g, respectively. Take 1 g each of amylase, cellulase, and hemicellulase, dissolve them in 500 ml of microbial culture solution, add them to the mixed material, and t...
Embodiment 2
[0041] The treatment of preparing straw fermented feed is the same as that in Example 1; it should be noted that the type of enzymes can be added without amylase, and can be added with cellulase and hemicellulase; the amount of enzymes can be increased appropriately, with 0.2 % is appropriate, and the high temperature pretreatment time is 2 days. The index of this feed product is: protein 20%-30%, oligosaccharide 2%-5%. The feed can be fed to ruminants such as cattle and sheep.
Embodiment 3
[0043] The process of preparing the fermented feed is the same as in Example 1; it should be noted that the corn bran and germ meal should be crushed to below 80 mesh in advance; The protein feed can be fed to fish.
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