Method for screening drought-enduring rhododendron germ plasma

A rhododendron germplasm technology, applied in seed and rhizome treatment, application, agriculture, etc., can solve the problems of low drought tolerance and screening of rhododendron drought-tolerant germplasm, etc.

Inactive Publication Date: 2015-04-29
JIANGSU ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, the research on Rhododendron is mostly focused on the introduction, domestication, propagation and cultivation, and there are few research reports on drought tolerance. It is still unknown how to use seeds as materials to screen the drought-tolerant germplasm of Rhododendron.

Method used

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Examples

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Embodiment Construction

[0019] For further understanding content of the invention and effect of the present invention, hereby cite the following examples, and the experimental methods used in the examples are conventional methods unless otherwise specified:

[0020] 1. Seed preparation: After storing the capsules of Rhododendron dabai, Rhododendron large fruit, Rhododendron dewdrops, Mayinhua, Rhododendron brocade, and Rhododendron yunnanensis for 6 months, remove the cracked or half-cracked capsules after they are fully dried, and remove the seeds with a sieve. Miscellaneous screening, microscopic observation method to remove shriveled seeds, and select plump and mature healthy seeds with a clarity of 100%.

[0021] 2. Disinfection of seeds: Soak dry seeds in 10% sodium hypochlorite or 0.5% potassium permanganate solution for 10 minutes, rinse with distilled water 3-4 times, and dry them with filter paper for later use.

[0022] 3. Seed germination: Put 6 parts of sterilized seeds into petri dishes ...

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Abstract

The invention discloses a method for screening drought-enduring rhododendron germ plasma. The method for screening the drought-enduring rhododendron germ plasma is characterized in that seeds are taken as an object, polyethylene glycol 6000 (PEG-6000) is utilized for simulating drought stress, a culture dish filter paper method is adopted for carrying out cultivation, and relative germination rate is selected as a drought-enduring index for carrying out screening; and the method for screening the drought-enduring rhododendron germ plasma comprises the following concrete steps: (1) selecting seeds and disinfecting; (2) simulating seed germination and cultivation under the drought stress condition by utilizing 15% PEG-6000; (2) carrying out seed germination and cultivation with distilled water (for contrast); and (4) determining the drought-enduring index relative germination rate, and screening drought-enduring germ plasma. The method for screening the drought-enduring rhododendron germ plasma can be used for screening a drought-enduring rhododendron germ plasma resource and is simple, easy, rapid and rapid, and a foundation is laid for breeding of drought-enduring rhododendron and evaluation of rhododendron germ plasma resource.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for screening rhododendron drought-tolerant germplasm. Background technique [0002] Rhododendron is a general term for plants of the genus Rhododendron in the family Ericaceae. Its germplasm resources are extremely rich. At present, there are nearly a thousand species and tens of thousands of cultivated varieties. It has high ornamental value and Its application value is one of the top ten famous flowers in my country and one of the three major alpine flowers in the world. It is also an important flower species for potted plants and landscaping, and occupies a very important position in the world's flower industry. The rhododendron root system is as thin as a hair and is very sensitive to moisture. Most rhododendron germplasms are afraid of drought, so the growth of rhododendron is most often affected by drought factors in the environment in field growth, lands...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 李畅陈璐苏家乐刘晓青陈尚平何丽斯肖政
Owner JIANGSU ACAD OF AGRI SCI
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