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Gene single nucleotide polymorphic sites related to asthma, kit for treating asthma and application of kit

A single nucleotide polymorphism and kit technology, applied in the fields of molecular biology and disease diagnosis, to achieve the effects of excellent specificity, increased risk, and convenient detection and operation

Inactive Publication Date: 2015-04-22
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Finally, the following conclusions were drawn: the asthma susceptibility gene prediction model of Han children was preliminarily established. Composed of nine single nucleotide polymorphism sites, it has good predictive performance for asthma, but the model still needs to be further improved

Method used

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  • Gene single nucleotide polymorphic sites related to asthma, kit for treating asthma and application of kit
  • Gene single nucleotide polymorphic sites related to asthma, kit for treating asthma and application of kit
  • Gene single nucleotide polymorphic sites related to asthma, kit for treating asthma and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1 Materials and methods

[0045] 1.1 Materials

[0046] 1.1.1 Research object

[0047] (1) Asthma case group: From 2007 to 2013, 1000 children with asthma from the asthma clinic of Xinhua Hospital, 503 males and 497 females, aged 3-12 years. All met the diagnostic criteria for childhood asthma revised by the Global Initiative for Asthma (GINA) committee in 2006: frequent wheezing episodes (or traceable to certain allergens or stimulating factors); Wheezing mainly in the gaseous phase, prolonged expiratory phase; bronchodilators have obvious curative effect, and there is reversible airflow limitation; other diseases that cause wheezing, chest tightness and cough are excluded. Because children with respiratory syncytial virus infection often suffer from wheezing, the lower age limit for enrollment was 3 years old. 76% of the enrolled children with IgE data had their total IgE levels above 400IU / ml.

[0048](2) Normal control group: 1000 healthy students from Shanghai ...

Embodiment 2

[0166] When evaluating the risk of asthma for a specific individual (the individual belongs to a child of the Han nationality in China), the following methods should be followed:

[0167] 1. Sample collection: it can be blood, saliva, oral mucosa, hair or tissue of the individual.

[0168] 2. Extract the genomic DNA of the specimen.

[0169] 3. Through direct sequencing; or the combination of polymerase chain reaction and restriction fragment length polymorphism analysis; or the combination of polymerase chain reaction and direct sequencing; or any method of SNP typing to obtain individual gene single Nucleotide sequences of nucleotide polymorphism sites IL13 R110Q, IL4-590C>T, ADRB2 R16G and FcER1 E237G.

[0170] 4. Compare the sequence of the single nucleotide polymorphism site of each gene with Table 5 of Example 1 to find out the probability of the individual suffering from asthma.

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Abstract

The invention relates to gene single nucleotide polymorphic sites related to asthma, a kit for treating asthma and an application of the kit. By carrying out genetic typing and single point and high order interaction and multi-site synergistic effect analysis on nine gene single nucleotide polymorphic sites, the result verifies that IL13 R110Q G, IL4-590C>T T, ADRB2 R16G A and FcER1B E237G G which are related to asthmatic pathogenesis of Chinese Han children are respectively risk alleles, and the risk factor of suffering from asthma by carrying four risk allele homozygotes is greatly improved; IL13 R110Q G, IL4-590C>T T, ADRB2 R16G A and FcER1B E237G G have a remarkable synergistic effect on risk of asthmatic pathogenesis. A reagent for detecting the four sites can be used for predicting asthma of Chinese Han children.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and disease diagnosis, in particular to gene single nucleotide polymorphism sites related to asthma, a kit for predicting asthma and applications thereof. Background technique [0002] Children's asthma is the most common respiratory disease that seriously affects children's physical and mental health. In recent years, the prevalence and mortality of children's asthma have been on the rise. In 1990, the national survey on the prevalence of asthma among children aged 0-14 was 0.91%. , It has risen to 1.5% in 2000, and this figure means that there are more than 10 million children with asthma in my country. Existing studies have confirmed that asthma is a chronic airway inflammatory disease. Due to the persistence of this chronic inflammatory response, the airway is in a state of hyperresponse, and symptoms will recur when exposed to triggers. The clinical treatment is mainly based on the...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 鲍一笑华丽
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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