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Acinetobacter baumannii and method for producing laccase and application thereof

A technology of Acinetobacter baumannii and laccase, which is applied in the biological field to achieve the effects of good stability, fast growth and simple operation

Inactive Publication Date: 2015-04-22
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the ability of Acinetobacter baumannii to produce laccase

Method used

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  • Acinetobacter baumannii and method for producing laccase and application thereof
  • Acinetobacter baumannii and method for producing laccase and application thereof
  • Acinetobacter baumannii and method for producing laccase and application thereof

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Experimental program
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Effect test

Embodiment 1

[0038] Laccase-producing Acinetobacter baumannii LMUDac, the Acinetobacter baumannii LMUDac was submitted for deposit on May 7, 2014, and the deposit number is: CCTCC NO: M2014189; depository unit: China Typical Culture Collection Center; Deposit Address: Wuhan University, Wuhan, China; Zip Code: 430072.

[0039] The method for isolating Acinetobacter baumannii is as follows: Take 1 g of soil samples from the outfall of Guangzhou Yihai Kerry Co., Ltd., add to the solution containing CuSO 4 In 100ml of LB liquid medium with a mass concentration of 0.05%, carry out enrichment culture in a 37°C incubator for 48 hours, use sterile water to carry out gradient dilution, and then use solid LB liquid medium to separate a single colony by dilution plate method. The ethanol solution of 1% mass guaiacol and the ethanol solution of 1% mass α-naphthol were used for color reaction, and pink or gray-black colonies were selected for repeated streak screening to obtain pure colonies, which wer...

Embodiment 2

[0041] A laccase obtained by:

[0042] 1) Culture medium treatment: fill the culture medium in the Erlenmeyer flask and the test tube respectively, adjust the pH value of the culture medium to 6, sterilize at 120°C for 25 minutes, and set aside after sterilization;

[0043] 2) Prepare copper sulfate solution: prepare 4% copper sulfate solution, sterilize at 125°C for 15 minutes, and set aside after sterilization;

[0044] 3) Inoculation: Inoculate Acinetobacter baumannii in the test tube of step 1), cultivate in a 32°C incubator for 25 hours, and then inoculate the bacteria in the test tube into the Erlenmeyer flask of step 1) according to the inoculation amount of 5%. Medium; the triangular flask was placed in a shaker incubator for culture, the culture conditions were 37°C, the shaker speed was 150r / min, and cultured for 25 hours;

[0045] 4) Fermentation culture: Then add the copper sulfate solution prepared in step 2) to the Erlenmeyer flask, so that the concentration of co...

Embodiment 3

[0054] A laccase obtained by:

[0055] 1) Culture medium treatment: fill the culture medium in the Erlenmeyer flask and the test tube respectively, adjust the pH value of the culture medium to 8, sterilize at 125°C for 15 minutes, and set aside after sterilization;

[0056] 2) Prepare copper sulfate solution: prepare 4% copper sulfate solution, sterilize at 120° C. for 5 minutes, and set aside after sterilization;

[0057] 3) Inoculation: Inoculate Acinetobacter baumannii in the test tube of step 1), cultivate in an incubator at 45°C for 20 hours, and then inoculate the thalli in the test tube into the Erlenmeyer flask of step 1) according to the inoculation amount of 5%. Medium; the Erlenmeyer flask was placed in a shaker incubator for culture, the culture conditions were 47°C, the shaker speed was 250r / min, and cultured for 20 hours;

[0058] 4) Fermentation culture: Then add the copper sulfate solution prepared in step 2) to the Erlenmeyer flask, so that the concentration ...

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Abstract

The invention discloses acinetobacter baumannii and a method for producing laccase and an application thereof. The acinetobacter baumannii is submitted for preservation on May 7 in 2014 and has a preservation number of CCTCC NO:M201489. The invention also discloses a method for producing laccase by using acinetobacter baumannii and an application of laccase in dye decolorization. The acinetobacter baumannii disclosed by the invention is relatively high in activity, high in growth rate, good in stability and difficult in variation, and can be subjected to fermentation culture to obtain laccase; the method for producing laccase by using the acinetobacter baumannii is simple to operate and low in cost; the obtained laccase is high in activity; and when laccase produced by the method disclosed by the invention is combined with syringaldazine to serve as a decoloring solution, the decoloring solution is high in activity and relatively high in stability, and then the decoloring effect of laccase can be effectively improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a laccase-producing Acinetobacter baumannii and a method and application for producing laccase. Background technique [0002] Acinetobacter baumannii (Acinetobacter baumannii) is a non-fermenting gram-negative bacillus, which widely exists in nature and belongs to conditional pathogenic bacteria. Acinetobacter baumannii has capsules, pili, no spores and flagella; it has strong adhesion and is easy to attach to the surface of objects. Acinetobacter baumannii has a complex antigenic structure, including bacterial antigens and capsular antigens. According to different antigens, Acinetobacter baumannii can be divided into 34 serotypes. [0003] Laccase (1accase) is a copper-containing polyphenol oxidase, which contains 19 kinds of amino acids. Laccase has a certain sugar content. Fungal laccase is a glycoprotein composed of peptide chains, sugar 2+ Composed of three parts, the molecula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/02C12N9/96C12R1/01
CPCC09B67/0096C12N1/20C12N9/0061C12Y110/03002C12N1/205C12R2001/01
Inventor 刘锐陈英杰黄小丹刘岩
Owner ZHONGKAI UNIV OF AGRI & ENG
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