Composite microbial inoculant for controlling plant continuous-cropping diseases and preparation method and application thereof
A compound bacterial agent and strain technology, applied in the directions of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of improving stress resistance, disease resistance, and fertilizer utilization.
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Embodiment 1
[0066] 1.1 Strain fermentation process
[0067] The production process of the compound preparation of Trichoderma harzianum, Bacillus subtilis and Glioma pink to prevent and control heavy cropping diseases is to make powder by liquid submerged fermentation method. The main steps of the process are the activation of the original strain → the expansion of the strain → the cultivation of the seed tank → fermentation Tank cultivation→filling material→stirring→chain machine crushing→powder sieving→product testing→packaging.
[0068] 1.1.1 Trichoderma harzianum
[0069] The strain patent preservation number of Trichoderma harzianum is CGMCC NO.7861. The production process is as follows:
[0070] 1.1.1.1 Strain activation
[0071] Trichoderma harzianum culture medium preparation: potato 20%, glucose 2%, agar 2%. Use NaOH or HCl to adjust the pH value to 7.0-7.5, then divide it into Erlenmeyer flasks or test tubes, place it in a sterilizer, and sterilize it under 0.11MPa pressure ...
Embodiment 2
[0131] Embodiment 2 (application composite bacterial agent is to strawberry growth promoting and disease prevention effect test)
[0132] Purpose of the test: In order to verify the effect of increasing yield and preventing disease of this product in a large-scale demonstration application on strawberries, a field plot test of this fertilizer was carried out on strawberries.
[0133] Test site: Xiaofengying Green Vegetable Garden Professional Cooperative, Kangzhuang Town, Yanqing County, Beijing
[0134] The composite microbial agent of Example 1 is selected: the ratio of Trichoderma harzianum, Glioma pink, Bacillus subtilis, phosphate solubilizing bacteria and potassium solubilizing bacteria is 1:1:3:2:2, and the bacterial content of the composite bacterial agent is, Bacteria content ≥ 500 million / g.
[0135] Tested variety: Strawberry variety "Hongyan"
[0136] Test method: The application method is hole application. There are 4 treatments in this test, namely: a1 conventi...
Embodiment 3
[0142] Embodiment 3 (application composite bacterial agent is to sugar beet root rot control effect and the test of increasing yield and increasing sugar)
[0143] Purpose of the test: In order to verify the effect of increasing yield and controlling root rot of this product in a large-scale demonstration application on sugar beet, a field test of this fertilizer was carried out on sugar beet.
[0144] Test site: sugar beet test field in Liangjia Village, Shangdu County, Ulanqab City, Inner Mongolia (heavy cropping field)
[0145] The composite microbial agent of Example 1 is selected: the ratio of Trichoderma harzianum, Glioma pink, Bacillus subtilis, phosphate solubilizing bacteria and potassium solubilizing bacteria is 1:1:3:2:2, and the bacterial content of the composite bacterial agent is, Bacteria content ≥ 500 million / g.
[0146] Test method: The application method is base application, and the treatments are compound bacterial agent and blank control. Each treatment is...
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