Device for detecting density-dependent growth inhibition of cells and application thereof

Abstract

The invention discloses a device for detecting the density-dependent growth inhibition of cells. The device comprises a culture vessel and 6-10 transparent plastic building blocks; the building blocks are shaped like fans, can be assembled together to form a cylinder and are fixed in the center area of the culture vessel; the area in the culture vessel, except the area occupied by the cylinder, serves as a cell culture observation area; 1 / 2 of the fan-like building blocks for forming the cylinder can be released from fixation, and 1 / 2 of the fan-like building blocks are provided with inclined surfaces which extend to the bottom surface of the culture vessel from the outer edges of the top surfaces of the fan-like building blocks; the outer edge of the top surface of each fan-like building block keeps a distance of 0.5-1.5mm with the bottom surface of the culture vessel, and included angles of 15-42 degrees are formed between the inclined surfaces and the bottom surface of the culture vessel. The invention further discloses application of the device to the dynamic observation of growth and contact inhibition of cells to be detected and the identification of normal cells and malignant tumour cells. The device is a tool provided for experiment teaching to detect the contact inhibition of cells intuitively, conveniently, flexibly and accurately.

Description

technical field [0001] The invention relates to a cell proliferation regulation detection device and application, in particular to a cell density-dependent growth inhibition detection device and application. Background technique [0002] Cells stop dividing when they come into contact with each other during growth. Since growth inhibition occurs when growth factors in the medium are depleted, the phenomenon that normal cells inhibit division due to mutual contact is also called density-dependent inhibition of growth. Malignant cells cultured under the same conditions lose their sensitivity to density-dependent growth inhibition, so they do not stop growing when they form a single layer, but accumulate on each other to form multi-layered growth aggregates, which also shows that The growth and division of malignant cells are out of control, and the signals that regulate the normal growth and division of cells no longer work on malignant cells. Therefore, using a specific det...

AI Technical Summary

Problems solved by technology

[0003] At present, instructing students to understand cancer cells in cell experiments is still limited to slices and static cell morphology observations. Teachers can only instill more advanced theories to students orally or in the form of model diagrams or pictures, and cannot be displayed in a dynamic form. No device has been reported for the use of detection devices, especially the use of specific vessels for the visual display of cell density-dependent growth inhibition or the difference between normal cells and malignant tumor cells

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