Microencapsulation preparation method of rhodococcus B7740 carotenoids
A carotenoid and Rhodococcus technology, which is applied in the directions of food preparation, food forming, application, etc., can solve the problems of easy oxidative degradation, light irradiation, instability and sensitivity of carotenoids, and achieves avoiding adverse effects, reducing production costs, and achieving high yields. rate effect
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Embodiment 1
[0036] A method for preparing rhodococcus B7740 carotenoid microcapsules, the steps are:
[0037] A. Weigh 0.5g of Rhodococcus B7740 freeze-dried bacterial powder (provided by Zhejiang Wanli University) and mix it with 10mL of 1mg / mL lysozyme (20000U / mg; biochemical reagent Sinopharm Chemical Reagent Co., Ltd.), break the cell wall in a 37°C water bath for 1 hour, and add 10mL Mix with saturated zinc acetate to precipitate protein, centrifuge at 6000r / min for 10min, discard the supernatant, and extract once or twice or three times with methanol / dichloromethane (8:2, v / v) at room temperature (20-25°C). A carotenoid extract is obtained.
[0038] B. Rotary evaporation of the carotenoid extract until viscous, adding 10 mL of soybean oil, and continued rotary evaporation to remove the organic solvent.
[0039]C. Weigh 1.5g chitosan and 3g gum arabic respectively, stir the gum arabic in hot water at 60°C to dissolve, cool to 20°C, adjust the pH to 3.6 with glacial acetic acid, and ...
Embodiment 2
[0046] A method for preparing microcapsules of rhodococcus carotenoid extract, the steps of which are:
[0047] A. Weigh 0.5g of Rhodococcus B7740 freeze-dried bacterial powder (provided by Zhejiang Wanli University) and mix it with 10mL 1mg / mL lysozyme (20000U / mg; Biochemical Reagent Sinopharm Chemical Reagent Co., Ltd.), break the cell wall in 37℃ water bath for 1h, add Mix 10mL saturated zinc acetate to precipitate protein, centrifuge at 6000r / min for 10min, discard the supernatant, and extract once or twice or three times with methanol / dichloromethane (8:2, v / v) at room temperature (20-25°C) , to obtain a carotenoid extract.
[0048] B. Rotary evaporation of the carotenoid extract until viscous, adding 10 mL of soybean oil, and continued rotary evaporation to remove the organic solvent.
[0049] C. Weigh 1g of chitosan and 3g of gum arabic respectively, stir the gum arabic in hot water at 60°C to dissolve it, cool to 20°C, adjust the pH to 3.6 with glacial acetic acid, an...
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