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Applications of dihydrochalcone and dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin

A technology of dihydrochalcone and active ingredients, which is applied in the field of application of dihydrochalcone and its glycosides as active ingredients for whitening and improving dull skin, and can solve the problem of single ingredients, no prevention or treatment of melanin, and improvement of skin. Issues such as reports in food, beverages, cosmetics, or pharmaceuticals

Active Publication Date: 2015-04-08
FOSHAN GOLDEN HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dihydrochalcone and its glycoside compounds involved in the present invention have definite and single ingredients, among which hesperetin dihydrochalcone (C) and naringin dihydrochalcone (D) are known to have exfoliating and Anti-acne effect (refer to Patent Document 6), hesperetin dihydrochalcone (C) is applied to the skin for anti-inflammatory and anti-allergy (refer to Patent Document 7); but currently there is no dihydrochalcone and Reports that its glycosides are used in foodstuffs, cosmetics or pharmaceuticals for preventing or treating melanin, improving skin quality

Method used

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  • Applications of dihydrochalcone and dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin
  • Applications of dihydrochalcone and dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin
  • Applications of dihydrochalcone and dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Tyrosinase Inhibition Test

[0035] 1. Determination of tyrosinase activity

[0036] 15 μL 5% DMSO solution, 60 μL 0.25 mmol / L L-Dopa, 195 μL pH 6.8 phosphate buffer solution, shake well. After incubation at 37°C for 5 min, 30 μL of tyrosinase solution was added and mixed immediately. Measure the absorbance value at 475 nm with a multi-functional microplate reader at a constant temperature of 37°C, make three parallel samples for each sample, and use the system without enzyme as a blank to draw a straight line of the increase of absorbance with time, from the slope of the straight line Enzyme activity A 0 .

[0037] 2. Determination of tyrosinase enzyme activity under the action of samples

[0038] 15 μL samples of different concentrations (5% DMSO), 60 μL 0.25 mmol / L L-Dopa, 195 μL pH 6.8 phosphate buffer solution, shake well. After incubating at 37°C for 5 minutes, add 30 μL of tyrosinase solution and mix immediately. Measure the absorbance value at 475 nm with ...

Embodiment 2

[0048] Inhibition of melanin production in B16 melanoma cells

[0049] Using NaOH cracking method. The day before the test, the cells were subcultured to make the confluence 30%-50%, and the DMEM-high glucose medium was used for the test. For example, a 6-well plate is used, the diameter of each well is 34.8mm, and the growth area is 9.5cm 2 . The volume of culture medium is 2000 μL. Add samples for processing the next day, when the confluence reaches 80%-90%, the cell volume is 1×10 6 / hole. When the eyes see obvious color changes in the medium, and the microscope observes black precipitates in the cells. After about 48 hours, the cells were collected for detection. Harvest 1×10 6 Wash the cells twice with PBS, add 200 μL redistilled water to suspend the precipitate, add 1 mL of ethanol:ether (1:1) mixture, let stand at room temperature for 20 min, centrifuge at 3000 r / min for 5 min, and add the precipitate 1 mol / L NaOH 1 mL (containing 10% DMSO), incubate in a water ...

Embodiment 4

[0055] Preparation of gel-like peel-off mask

[0056] In 65g of pure water, add 5g of carboxymethylcellulose and 10g of polyvinyl alcohol, dissolve while heating, add 0.1g of hesperidin dihydrochalcone and 19g of ethanol at the same time, add a small amount of citrus essence, Makes into a gel-like peel-off mask.

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Abstract

The invention discloses applications of dihydrochalcone and a dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin. Structural formula of the dihydrochalcone glucoside compound is represented by formula (I), wherein R1, and R2 are used for representing H, OH, or methoxy respectively, R3 is used for representing H, glucosyl, rutinose group, or neohesperidose group. The dihydrochalcone glucoside compound can be used as food, cosmetics, or pharmaceuticals used for preventing or treating melanin pigmentation, and improving skin; and is excellent in effects.

Description

technical field [0001] The present invention relates to using dihydrochalcone and its glycoside compounds as active ingredients for whitening and improving dark skin quality, and in particular to said dihydrochalcone and its glycoside compounds as tyrosinase inhibitors, which can effectively block melanin Generate and promote the reduction of pigmentation, and be used in the preparation of foodstuffs, cosmetics or pharmaceuticals for preventing or treating melanin and improving skin quality. Background technique [0002] In today's society, the pace of life and work continues to accelerate, environmental pollution and the destruction of the ozone layer cause the formation and metabolism of melanin to become chaotic, resulting in an increasing number of people suffering from various black spots; activated oxygen produced and ingested from inside and outside the body is oxidized Problems such as skin aging caused by reactions and skin coloration caused by ultraviolet rays are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K8/35A61Q19/02A61K31/7034A61K31/12A61P17/00A23L1/30A23L33/10
Inventor 周金林卢宇靖黄宝华
Owner FOSHAN GOLDEN HEALTH TECH CO LTD
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