Method of synthesizing poly 3-hydracrylic acid by utilizing recombinant Escherichia coli

Abstract

The invention relates to a method of synthesizing poly 3-hydracrylic acid by utilizing recombinant Escherichia coli and protects a recombinant Escherichia coli strain, a construction method of the recombinant Escherichia coli strain and a method of synthesizing the poly 3-hydracrylic acid by using the recombinant Escherichia coli strain by virtue of a biological method. The method has the beneficial effects that in the metabolic pathway of biologic synthesis of P3HP with glycerol serving as a substrate, NAD+, serving as coenzyme, can generate a great deal of NADH when used for catalyzing 3-hydroxy-propionaldehyde to produce 3HP; the oxidation-reduction disequilibrium in a recombinant strain, caused by increase of NADH is capable of inhibiting further increase of the yield of P3HP, and NADH can be consumed in the process that the recombinant Escherichia coli strain metabolizes glycerol to generate 1,3-PDO, and therefore synthesis of 1,3-PDO is introduced into the synthesis pathway of P3HP, the in-vivo reducing power of the strain can be balanced, the plasmid loss rate is effectively reduced, and the plasmid stability is kept.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

AI Technical Summary

Benefits of technology

This patented method allows for increased production of pigmented acid (PA) without consuming too much or losing important enzymes like phosphatases during cell growth. It also introduces an intermediate product called 2,3-pDPG, which helps keep it stable when being used instead of producing PA from other compounds such as glucose. Overall this new technique improves efficiency and yield of chemical processes involved in making certain types of molecules.

Problems solved by technology

Technological Problem: Current methods for making poly(3- hydroxy propional acid)) require costly starting materials like beta lactic acid or other similar ones. Additionally, current techniques involve complicated processes involving multiple steps leading to low yields and poor quality products.

Images