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Process for extracting anthocyanin from nitraria tangutorum bobr pomace

A technology of white thorn fruit and anthocyanin, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve problems such as not being used, and achieve the effects of lowering blood sugar, simple extraction method and high extraction rate.

Inactive Publication Date: 2015-03-25
QINGDAO ZHONGREN ZHIYE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of utilizing Nitraria japonica fruit juice and seeds, the pomace therein is discarded and has not been utilized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: get 1000g Nitraria pomace, carry out enzymatic hydrolysis treatment, in the enzyme preparation used, comprise cellulase, and the addition of cellulase is 0.15% of Nitraria pomace weight, enzymatic hydrolysis time 8min, temperature 20 ℃, and use sodium acetate to adjust the pH value in the enzymatic hydrolysis process to be 3; add 30% ethanol ultrasonic extraction in the material system after enzymatic hydrolysis, the mass volume ratio (g:mL) of Nitraria pomace and ethanol is 1 : 10, extraction time 15min, temperature 45°C, ultrasonic power 150w, and during the ultrasonic extraction process, sodium acetate was used to maintain an acidic environment with a pH value of 3 in the system; Purify the anthocyanins in the centrifuged liquid with resin, the adsorption capacity of the resin is 50mg / g, the elution rate is 80%, and then eluted with 40% ethanol, and the extract and eluate are combined and collected at a temperature of 40 ℃, under the condition of 60kPa ...

Embodiment 2

[0012] Embodiment 2: get 1000g Nitraria pomace, carry out enzymatic hydrolysis treatment, the enzyme preparation used is cellulase, and the addition of cellulase is 0.18% of Nitraria pomace weight, enzymatic hydrolysis time 10min, temperature 30 ℃, and use sodium acetate to adjust the pH value in the enzymatic hydrolysis process to 4; add 35% ethanol to the material system after enzymatic hydrolysis for ultrasonic extraction, and the mass volume ratio (g: mL) of Nitraria pomace and ethanol is 1: 15. The extraction time is 20 minutes, the temperature is 50°C, and the ultrasonic power is 200w. During the ultrasonic extraction process, sodium acetate is used to maintain an acidic environment with a pH value of 4 in the system; The resin purifies the anthocyanins in the centrifuged liquid, and the resin adsorption capacity is 60mg /

[0013] g, the elution rate is 70%, and then eluted with 50% ethanol, and the extract and eluate are collected together, and at a temperature of 50°C ...

Embodiment 3

[0014] Embodiment 3: get 1000g Nitraria pomace, carry out enzymatic hydrolysis treatment, in the enzyme preparation used, comprise cellulase, and the addition of cellulase is 0.2% of raw material weight, enzymatic hydrolysis time 14min, temperature 40 ℃, And use sodium acetate to adjust the pH value in the enzymatic hydrolysis process to 4.6; add 40% ethanol to the material system after enzymatic hydrolysis for ultrasonic extraction. , the temperature is 55°C, the ultrasonic power is 250w, and during the ultrasonic extraction process, sodium acetate is used to maintain an acidic environment with a pH value of 4.6 in the system; The anthocyanins in the liquid are purified, the resin adsorption capacity is 70mg / g, the elution rate is 90%, and then eluted with 60% ethanol, and the extract and eluate are combined and collected, at a temperature of 60°C and a pressure of 88kPa , concentrated under reduced pressure to recover ethanol to obtain a concentrate, and then put the concent...

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Abstract

The invention relates to a process for extracting anthocyanin from nitraria tangutorum bobr pomace. The process comprises the working procedures of enzymolysis, extraction, separation, purification, elution, concentration and drying. The method disclosed by the invention is simple, the extraction rate is up to 75%, resources can be comprehensively utilized, industrialized preparation is promoted, and a novel natural plant raw material can also be provided for medicines as well as health foods for preventing tumours, reducing blood sugar, and protecting and benefiting bile.

Description

technical field [0001] The invention relates to a process for extracting natural pigments, in particular to a process for extracting anthocyanins from Nitraria pomace. Background technique [0002] Nitraria L. is a small deciduous shrub of the genus Nitraria L. in the family Zygophyllaceae. It is a common wild saline-alkali plant in Northwest my country, which has high economic, ecological and medicinal value. The fruit is rich in vitamins, amino acids, polysaccharides and other nutritional active ingredients. [0003] Components, especially amino acids, are easily absorbed and utilized by the human body, and the pomace contains an average of 0.37% anthocyanins. [0004] Anthocyanins are water-soluble pigments, which belong to flavonoids and mostly exist in the form of glycosides, also known as anthocyanins. As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have significant pharmacological effects. They have great application potential i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C07H17/065C07H1/08
CPCC07H17/065C07D311/62C07H1/08
Inventor 侯维
Owner QINGDAO ZHONGREN ZHIYE BIOTECH
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