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Growth factor nanotube slow-release system used for osseointegration and preparation method and application thereof

A growth factor and nanotube technology, used in medical science, surgery, etc., can solve the problems that the biological activity of growth factors cannot be maintained for a long time, the research has not been reported in the literature, and there is explosive release. The amount of drug and the effect of promoting osseointegration

Inactive Publication Date: 2015-03-25
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to further improve the repair effect of nanotube coating on the titanium surface, the hollow tubular structure and high specific surface area of ​​nanotubes can be used for drug delivery to synergistically promote osseointegration, but this aspect has not been reported in the literature.
[0003] Growth factors that can be used to promote osseointegration include recombinant human platelet-derived growth factor (rhPDGF-BB), bone morphogenetic proteins (BMPs), basic fibroblast growth factor (bFGF), vascular epidermal growth factor (VEGF), Nel-like I At present, growth factors are often immobilized on the surface of implants by direct physical adsorption or coating with materials such as chitosan. However, these methods have the following disadvantages: slow-release effect is poor, and there is often explosive release; growth factor The biological activity of the implant cannot be maintained for a long time; additional slow-release materials may also cover the micro-nano structure on the surface of the implant and eliminate its biological performance

Method used

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  • Growth factor nanotube slow-release system used for osseointegration and preparation method and application thereof
  • Growth factor nanotube slow-release system used for osseointegration and preparation method and application thereof
  • Growth factor nanotube slow-release system used for osseointegration and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Nanotube modification and drug loading on the surface of titanium sheet

[0029] Step 1. Nanotube modification on the surface of titanium sheet

[0030] A titanium alloy material with a size of 10 × 10 × 1 mm is placed in an ethanol solution for ultrasonic cleaning for 30 minutes; then replaced with deionized water and ultrasonic cleaning is continued for 30 minutes. Repeat the operation 2 times; place the cleaned titanium sheet Acid etching in 5 wt% oxalic acid solution at 100°C for 2 hours; finally, it is placed in 1.0 wt% hydrofluoric acid electrolyte using constant current direct current anodizing (voltage 20V) for 30 minutes to prepare nanometers with a diameter of about 70nm Tube coating.

[0031] Step 2: Vacuum suction promotes the loading of rhPDGF-BB with nanotubes on the surface of the titanium sheet

[0032] In the commercial GEM 21S kit, the concentration of the original rhPDGF-BB solution is 300μg / ml, and it is diluted to a working concentration of 50μg...

Embodiment 2

[0033] Example 2. Nanotube modification and drug loading on the surface of titanium sheet

[0034] Step 1. Nanotube modification on the surface of titanium sheet

[0035] Titanium alloy material with a size of 10 × 10 × 1 mm is placed in an ethanol solution for ultrasonic cleaning for 30 minutes; then replaced with deionized water and ultrasonic cleaning is continued for 30 minutes. Repeat the operation 2 times; place the cleaned titanium sheet Acid etching in 5 wt% oxalic acid solution at 100°C for 2 hours; finally placed in 1.0 wt% hydrofluoric acid electrolyte and used constant current direct current anodization (voltage 20V) for 30 minutes to prepare nanometers with a diameter of about 70nm Tube coating.

[0036] Step 2: Vacuum suction promotes the loading of rhPDGF-BB with nanotubes on the surface of the titanium sheet

[0037] In the commercial GEM 21S kit, the concentration of the original rhPDGF-BB solution is 300μg / ml. Use sterile medical water to dilute it to a working conc...

Embodiment 3

[0038] Example 3. Nanotube modification and drug loading on the surface of titanium sheet

[0039] Step 1. Nanotube modification on the surface of titanium sheet

[0040] A titanium alloy material with a size of 10 × 10 × 1 mm is placed in an ethanol solution for ultrasonic cleaning for 30 minutes; then replaced with deionized water and ultrasonic cleaning is continued for 30 minutes. Repeat the operation 2 times; place the cleaned titanium sheet Acid etching in 5 wt% oxalic acid solution at 100°C for 2 hours; finally placed in 1.0 wt% hydrofluoric acid electrolyte and used constant current direct current anodization (voltage 20V) for 30 minutes to prepare nanometers with a diameter of about 70nm Tube coating.

[0041] Step 2: Vacuum suction promotes the loading of rhPDGF-BB with nanotubes on the surface of the titanium sheet

[0042] In the commercial GEM 21S kit, the concentration of the rhPDGF-BB stock solution is 300μg / ml, and 50μl of this solution is dripped onto the nanotube co...

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Abstract

The invention discloses a growth factor nanotube slow-release system used for osseointegration and a preparation method and application thereof, the nanotube slow-release system includes a titanium alloy implant, a titanium dioxide nanotube coating is prepared on the titanium alloy implant surface by the method of anodic oxidation, and the titanium alloy implant is loaded with a growth factor. The growth factor nanotube slow-release system has the advantages that: (1) the growth factor nanotube slow-release system can improve the nanotube drug loading amount by a simple and effective method of vacuum aspiration; (2) the invention also provides a rhPDGF- BB nanotube slow-release system which can significantly promote the osseointegration in bone osteoporosis state, rhPDGF-BB is approved by the FDA to be used in clinical osteanagenesis therapies, and clinical application of the nanotube slow-release system may be provided; and (3) including nanotube preparation, therowth factor nanotube slow-release system is in no need of special, complex and expensive equipment, simple in operation process, and conducive to the promotion and use.

Description

Technical field [0001] The invention relates to the field of bone tissue regeneration and osseointegration, in particular to a growth factor nanotube slow-release system applied to osseointegration, and a preparation method and application thereof. Background technique [0002] Osteoporosis is due to defects in the coupling of bone resorption and bone formation in the process of bone metabolism, resulting in decreased bone density and bone destruction, which seriously affects the speed and quality of bone repair and osseointegration; due to the lack of effective means to solve this problem Problem, osteoporosis is still listed as a contraindication for dental implant surgery. With the continuous development and maturity of nanotechnology, a variety of nano-modified coatings have been used to improve the osteoinductive properties on the surface of titanium implants. Among them, the nanotube coating prepared by anodizing technology not only has obvious regulation of stem cell osteo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/02A61L31/16C25D11/26
Inventor 张文杰蒋欣泉
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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