Sea cucumber flower extractive used for promoting postpartum uterus contraction
A technology of uterine contraction and sea cucumber flower, which is applied in the direction of echinoderm medical raw materials, drug combination, pill delivery, etc., can solve the problems that uterine contraction has not been reported.
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Embodiment 1
[0010] Embodiment 1, preparation has the sea cucumber flower extract that promotes postpartum uterine contraction:
[0011] Sea cucumber flowers (fresh and frozen) are provided by Dalian Bangchuidao Seafood Co., Ltd.
[0012] (1) Appropriate amount of sea cucumber flower tissue, homogenized;
[0013] (2) Add 1.5 times the volume of distilled water, and ultrasonically assist extraction at 30°C for 25 minutes;
[0014] (3) Stir and extract in a water bath at 30°C for 2 hours, centrifuge at 4000r / min for 5 minutes, take the supernatant, repeat the above operation for the residue, combine the filtrates, concentrate under reduced pressure to 1 / 3 volume, and freeze-dry to obtain sea cucumber flower extract.
Embodiment 2
[0015] Example 2: Effect of Sea Cucumber Flower Extract on Uterine Contraction of Isolated Mice
[0016] Preparation of isolated mouse uteri: 2 days before the experiment, non-pregnant female mice were intraperitoneally injected with estradiol valerate every day to improve the sensitivity of the uterus to drugs; the mice were killed quickly by cervical dislocation, and the uteri were taken out by laparotomy to make about 1.0 cm strips of uterine smooth muscle. Put the uterus ligated with silk thread into a Maxwellian bath filled with 20ml, fix one end of the silk thread ligated to the specimen fixing hook in the bath, and connect the other end to the tension transducer, and give the uterus a load of 1g. After the uterine contraction is stable, the normal contraction curve is recorded. Add 1 drop of motherwort aqueous solution (as a control) and various concentrations of sea cucumber flower extracts to each group, and after the uterine contraction is stable, use Biolap420E + ...
Embodiment 3
[0019] Embodiment 3: the preparation of sea cucumber flower extract tablet
[0020] prescription:
[0021] Sea cucumber flower extract 100mg
[0022] Lactose 100mg
[0023] Starch 295mg
[0025] Total 500mg
[0026] Weigh out each main component, mix sea cucumber flower extract powder through 80 mesh sieve, lactose and starch, granulate by wet method, dry, mix with magnesium stearate after integration, compress into tablets, and compress into 500mg per tablet tablet.
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