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Analysis and identification method on miRNA key target gene of blood fluke in specific growth period

A developmental stage and key gene technology, applied in the field of life medical science, can solve problems such as difficult to accurately determine key target genes

Inactive Publication Date: 2015-03-11
TONGJI UNIV
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Problems solved by technology

Therefore, the currently used miRNA identification method combining bioinformatics and in vitro experiments is difficult to accurately determine the key target gene regulated by miRNA in a certain developmental period, and the determination of the target gene will be crucial to understanding the function of the miRNA

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  • Analysis and identification method on miRNA key target gene of blood fluke in specific growth period
  • Analysis and identification method on miRNA key target gene of blood fluke in specific growth period
  • Analysis and identification method on miRNA key target gene of blood fluke in specific growth period

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Embodiment

[0050] A method for analyzing and identifying key target genes of Schistosoma japonicum miRNA at a specific developmental stage, comprising the following steps:

[0051] Step 1: Analysis of miRNA expression characteristic of Schistosoma japonicum at specific developmental stages:

[0052]Through Solexa analysis, compare the miRNA expression characteristics of Schistosoma japonicum in different developmental stages, and determine the characteristic miRNA of Schistosoma japonicum in specific developmental stages; Body miRNA expression profiles. Characteristic miRNAs differentially expressed in different periods were analyzed. Such as figure 1 . from figure 1 It can be seen that the miRNA expression profiles are different in different developmental stages of worms, suggesting that each stage has its own characteristics. For example, at 18 days after infection, the expression of miRNA-1c, miRNA-1b and miRNA-1a increased in single-sex (s18) or double-sex (d18) infected females...

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Abstract

The invention an analysis and identification method on the miRNA key target gene of blood fluke in the specific growth period, and belongs to the field of life medicine science. Bioinformatics is adopted to analyze the blood fluke genome so as to find certain miRNA target gene groups in the blood fluke genome. Then Solexa is utilized to analyze the blood fluke expression difference gene groups in the high expression period of miRNA and the low expression period of miRNA. The overlapped part between the blood fluke expression difference gene groups and the miRNA target gene groups is the candidate key gene groups. Then KEGG analysis is used to find the important genes in the candidate key gene groups so as to determine the key target gene group primarily. Then the miRNA mimic is utilized to carry out in-vitro experiments to further screen out the key target genes, and finally the target relationship between the miRNA and the key target genes is further confirmed through a luciferase reporter system. The establishment of the provided method can help the identification of the key target genes of miRNA in the specific period, help the disclosure of the modulation effect of miRNA, and provide an ideal route for miRNA function researches. At the same time valuable references are provided for the similar research methods on other species.

Description

technical field [0001] The invention belongs to the field of life medical sciences, and in particular relates to a method for analyzing and identifying key target genes of Schistosoma japonicum miRNA at a specific developmental stage. Background technique [0002] In organisms, miRNAs often have numerous target genes, and a gene often has multiple miRNA binding sites. In addition, miRNAs have different priorities in the regulation of different target genes in different stages of insect body development. Therefore, the currently used miRNA identification method combining bioinformatics and in vitro experiments is difficult to accurately determine the key target gene regulated by miRNA in a certain developmental period, and the determination of the target gene will be crucial to understanding the function of the miRNA. Contents of the invention [0003] The purpose of the present invention is to provide a method for analyzing and identifying key target genes of Schistosoma ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/158C12Q2600/178
Inventor 孙军王素文
Owner TONGJI UNIV
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