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Rapid screening method for pebrine resistant germplasms

A technology of silkworm microparticles and microsporidiosis, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve problems such as errors in breeding result data and differences in pathogen intake, and achieve prevention Effects of cross-infection, guarantee of reliability, improvement of repeatability and credibility

Inactive Publication Date: 2015-03-11
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] In order to solve the above problems, the present invention discloses a rapid screening method for silkworm microparticle disease-resistant germplasm to solve the problem of large differences in pathogen intake between individuals and breeding result data in the existing screening methods for silkworm microparticle resistance. There is a problem of error, and a rapid screening method for silkworm microparticle disease-resistant germplasm that strictly controls the intake of individual pathogens is provided. The results are more accurate and reliable, shortening the period of silkworm breeding screening for resistance to silkworm microparticle disease

Method used

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  • Rapid screening method for pebrine resistant germplasms

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Embodiment 1

[0024] The concrete steps of this embodiment are:

[0025] Step 1: Select several kinds of silkworms for testing, namely Gaobai, Fang 1, and Yan 7, and raise them to the third instar silkworms. Select silkworms of uniform size and put them in a 28-hole insect breeding box, and place each silkworm in a single hole;

[0026] Step 2: Prepare mulberry leaf discs with a diameter of 1.0 cm with a mulberry cutting tool, use a pipette gun to draw 3-7 μl of the original solution of No. 6 pieces / mL, put into the rearing box after drying, one piece per head;

[0027] Step 3: After 4 hours, select the silkworms that have eaten the mulberry leaves as the silkworms for further experiments. Each strain is divided into 3 groups, and each group has 3 repeated areas, 50 in each area, and continues to carry out individual rearing;

[0028] Step 4: After 12 days, perform microscopic examination of silkworms to investigate the number of infected heads of each strain;

[0029] Step 5: record the ...

Embodiment 2

[0031] The concrete steps of this embodiment are:

[0032] Step 1: Select several kinds of silkworms for testing, namely Gaobai, Fang1, and Yan7, and raise them to the 3rd instar silkworms. Select silkworms with uniform size and put them into different insect breeding boxes according to different strains, and carry out group breeding;

[0033] Step 2: Prepare mulberry leaf discs with a diameter of 1.0 cm with a mulberry cutting tool, use a pipette gun to draw 3-7 μl of the original solution of No. 6 pieces / mL, after drying, put them into the rearing box with the amount of one piece per head;

[0034] Step 3: After 4 hours, divide each strain into 3 groups, each group has 3 repeated areas, 50 heads in each area, and each 50 heads are group-raised;

[0035] Step 4: After 12 days, perform microscopic examination of silkworms to investigate the number of infected heads of each strain;

[0036] Step 5: record the infection rate of each group, and understand the resistance of vari...

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Abstract

The invention discloses a rapid screening method for pebrine resistant germplasms. The method comprises main steps as follows: 1, silkworms are fed to newly molted third instar larvas and are singly placed; 2, the silkworms are inoculated with round mulberry leaf blades coated with microsporidiosis stock solution; 3, grouping and zoning are performed; 4, silkworm smash and mirror checking is performed; and 5, the infection rate is calculated, and the resistant germplasms are screened. The pebrine resistance evaluation method aims to solve the problems that the pathogen intake amounts among individuals have larger differences and data of screening results have errors in the conventional method, and the rapid screening method for the pebrine resistant germplasms is stable and reliable.

Description

technical field [0001] The invention belongs to the field of sericulture cultivation and breeding, and in particular relates to a rapid screening method for silkworm microparticle disease-resistant germplasm. Background technique [0002] Bombyx mori microparticle disease is an infectious silkworm disease caused by Nosema bombycis. Microsporidium silkworm can not only infect silkworms by eating, but also infect silkworms from mother to offspring through embryo seeds, and then invade various tissues of silkworms, eventually leading to death of silkworms. Since there is no effective treatment so far, once the disease occurs, it will cause huge economic losses to sericulture production, especially silkworm egg production. Breeding disease-resistant varieties may become the most effective way to solve the silkworm microparticle blight. [0003] With the advancement of molecular biology technology, molecular markers and transgenic technology have been widely used in the breedin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/90
Inventor 杨琼邢东旭李丽罗国庆肖阳李庆荣叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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