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A method for extracting DHA algae oil from algae cells

A technology of algal cells and algal oil, which is applied in the production of fat/fat and fat production, can solve the problem of low DHA extraction rate, and achieve the effects of no organic solvent residue, improved extraction efficiency, and improved extraction rate.

Active Publication Date: 2017-12-01
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned extraction process is limited by the technical conditions of the existing wall-breaking process conditions, and the extraction rate of DHA is still low

Method used

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  • A method for extracting DHA algae oil from algae cells

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Effect test

Embodiment 1

[0042] A method for extracting DHA algae oil from Schizochytrium, comprising the steps of:

[0043] (1) After the Schizochytrium fermented liquid is centrifuged and spray-dried, the Schizochytrium dry powder is prepared, and then the Schizochytrium dry powder and absolute ethanol are mixed uniformly according to the mass-volume ratio of 1:5, and the unit is kg / L, stand still for 30min, then carry out high-pressure homogeneous wall breaking under the condition of pressure of 100Mpa, and cycle breaking the wall 3 times to obtain the broken wall ethanol solution;

[0044] (2) Evaporating the broken-wall ethanol solution prepared in step (1) at a temperature of 45°C and a pressure of -0.08Mpa in a low-temperature vacuum to remove ethanol to obtain broken-wall bacteria;

[0045] (3) Get the broken-walled thalline that step (2) makes, carry out CO 2 Supercritical extraction to obtain DHA algae oil.

[0046] The Schizochytrium fermented liquid in described step (1), preparation st...

Embodiment 2

[0059] A method for extracting DHA algae oil from Schizochytrium, comprising the steps of:

[0060] (1) After the Schizochytrium fermented liquid is centrifuged and spray-dried, the Schizochytrium dry powder is prepared, and then the Schizochytrium dry powder and absolute ethanol are mixed evenly according to the mass volume ratio of 1:6, and the unit is kg / L, stand still for 25min, then carry out high-pressure homogeneous wall breaking under the condition of pressure of 80Mpa, and circulate wall breaking 4 times to obtain broken wall ethanol solution;

[0061] (2) Evaporating the broken-wall ethanol solution prepared in step (1) at a temperature of 40°C and a pressure of -0.12Mpa in a low-temperature vacuum to remove ethanol to obtain broken-wall bacteria;

[0062] (3) Get the broken-walled thalline that step (2) makes, carry out CO 2 Supercritical extraction to obtain DHA algae oil.

[0063] The Schizochytrium fermented liquid in described step (1), preparation steps are ...

Embodiment 3

[0076] A method for extracting DHA algae oil from Schizochytrium, comprising the steps of:

[0077] (1) After the Schizochytrium fermented liquid is centrifuged and spray-dried, the Schizochytrium dry powder is prepared, and then the Schizochytrium dry powder and absolute ethanol are mixed uniformly according to the mass volume ratio of 1:4, and the unit is kg / L, stand still for 40min, then carry out high-pressure homogeneous wall breaking under the condition of pressure of 120Mpa, and circulate wall breaking 2 times to obtain broken wall ethanol solution;

[0078] (2) Evaporating the broken-wall ethanol solution prepared in step (1) at a temperature of 50°C and a pressure of -0.05Mpa in a low-temperature vacuum to remove ethanol to obtain broken-wall bacteria;

[0079] (3) Get the broken-walled thalline that step (2) makes, carry out CO 2 Supercritical extraction to obtain DHA algae oil.

[0080] The Schizochytrium fermented liquid in described step (1), preparation steps ...

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Abstract

The invention relates to a method for extracting DHA algae oil from algae cells. The method comprises the following steps: (1) centrifuging and spray-drying algae cell fermentation broth to prepare algae cell dry powder, then uniformly mixing the algae cell dry powder with absolute ethanol, and carrying out high-pressure homogenizing cell wall breaking to prepare a wall-broken ethanol solution; (2) distilling the wall-broken ethanol solution at a low temperature in vacuum, removing ethanol to prepare wall-broken bacteria; and (3) carrying out CO2 supercritical extraction on the wall-broken bacteria to obtain DHA algae oil. According to the method, the algae cell dry powder is soaked by the absolute ethanol before high-pressure homogenizing cell wall breaking, so that the extraction rate can be greatly increased.

Description

technical field [0001] The invention relates to a method for extracting DHA algae oil from algae cells, belonging to the technical field of health product preparation. Background technique [0002] Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid. Modern medicine has proved that DHA can promote the visual and intellectual development of infants and young children, and also has a positive preventive effect on cardiovascular system diseases, cancer, and inflammation. The production of DHA has a variety of sources, among which the production of DHA algae oil by microbial fermentation has the advantages of easy large-scale cultivation, high polyunsaturated fatty acid content, easy separation and purification, and good oxidation stability. Among the many DHA-producing microorganisms (including Schizochytrium, Thraustochytrium, Cryptodinophyllum, etc.), Schizochytrium has many advantages such as fast growth and high DHA content, and is one ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/04C11B1/00C11B1/10
CPCC11B1/00C11B1/04C11B1/104
Inventor 窦光朋霍文严黄磊黎丽魏巍邵先豹
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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