Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting DHA algae oil from algae cells

A technology of algae cells and algae oil, which is applied in the direction of fat oil/fat production, fat production, etc., can solve the problem of low extraction rate of DHA, achieve the effect of no organic solvent residue, improve extraction efficiency, and increase extraction rate

Active Publication Date: 2015-03-04
SHANDONG BAILONG CHUANGYUAN BIO TECH
View PDF8 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned extraction process is limited by the technical conditions of the existing wall-breaking process conditions, and the extraction rate of DHA is still low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting DHA algae oil from algae cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for extracting DHA algae oil from Schizochytrium, comprising the steps of:

[0043] (1) After the Schizochytrium fermented liquid is centrifuged and spray-dried, the Schizochytrium dry powder is prepared, and then the Schizochytrium dry powder and absolute ethanol are mixed evenly according to the mass volume ratio of 1:5, and the unit is kg / L, stand still for 30min, then carry out high-pressure homogeneous wall breaking under the condition of pressure of 100Mpa, and cycle breaking the wall 3 times to obtain the broken wall ethanol solution;

[0044] (2) Evaporating the broken-wall ethanol solution prepared in step (1) at a temperature of 45°C and a pressure of -0.08Mpa in a low-temperature vacuum to remove ethanol to obtain broken-wall bacteria;

[0045] (3) Get the broken-walled thalline that step (2) makes, carry out CO 2 Supercritical extraction to obtain DHA algae oil.

[0046] The Schizochytrium fermented liquid in described step (1), preparation steps...

Embodiment 2

[0059] A method for extracting DHA algae oil from Schizochytrium, comprising the steps of:

[0060] (1) After the Schizochytrium fermented liquid is centrifuged and spray-dried, the Schizochytrium dry powder is prepared, and then the Schizochytrium dry powder and absolute ethanol are mixed evenly according to the mass volume ratio of 1:6, and the unit is kg / L, stand still for 25min, then carry out high-pressure homogeneous wall breaking under the condition of pressure of 80Mpa, and circulate wall breaking 4 times to obtain broken wall ethanol solution;

[0061] (2) Evaporating the broken-wall ethanol solution prepared in step (1) at a temperature of 40°C and a pressure of -0.12Mpa in a low-temperature vacuum to remove ethanol to obtain broken-wall bacteria;

[0062] (3) Get the broken-walled thalline that step (2) makes, carry out CO 2 Supercritical extraction to obtain DHA algae oil.

[0063] The Schizochytrium fermented liquid in described step (1), preparation steps are ...

Embodiment 3

[0076] A method for extracting DHA algae oil from Schizochytrium, comprising the steps of:

[0077] (1) After the Schizochytrium fermented liquid is centrifuged and spray-dried, the Schizochytrium dry powder is prepared, and then the Schizochytrium dry powder and absolute ethanol are mixed uniformly according to the mass volume ratio of 1:4, and the unit is kg / L, stand still for 40min, then carry out high-pressure homogeneous wall breaking under the condition of pressure of 120Mpa, and circulate wall breaking 2 times to obtain broken wall ethanol solution;

[0078] (2) Evaporating the broken-wall ethanol solution prepared in step (1) at a temperature of 50°C and a pressure of -0.05Mpa in a low-temperature vacuum to remove ethanol to obtain broken-wall bacteria;

[0079] (3) Get the broken-walled thalline that step (2) makes, carry out CO 2 Supercritical extraction to obtain DHA algae oil.

[0080] The Schizochytrium fermented liquid in described step (1), preparation steps ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting DHA algae oil from algae cells. The method comprises the following steps: (1) centrifuging and spray-drying algae cell fermentation broth to prepare algae cell dry powder, then uniformly mixing the algae cell dry powder with absolute ethanol, and carrying out high-pressure homogenizing cell wall breaking to prepare a wall-broken ethanol solution; (2) distilling the wall-broken ethanol solution at a low temperature in vacuum, removing ethanol to prepare wall-broken bacteria; and (3) carrying out CO2 supercritical extraction on the wall-broken bacteria to obtain DHA algae oil. According to the method, the algae cell dry powder is soaked by the absolute ethanol before high-pressure homogenizing cell wall breaking, so that the extraction rate can be greatly increased.

Description

technical field [0001] The invention relates to a method for extracting DHA algae oil from algae cells, belonging to the technical field of health product preparation. Background technique [0002] Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid. Modern medicine has proved that DHA can promote the visual and intellectual development of infants and young children, and also has a positive preventive effect on cardiovascular system diseases, cancer, and inflammation. The production of DHA has a variety of sources, among which the production of DHA algae oil by microbial fermentation has the advantages of easy large-scale cultivation, high polyunsaturated fatty acid content, easy separation and purification, and good oxidation stability. Among the many DHA-producing microorganisms (including Schizochytrium, Thraustochytrium, Cryptodinophyllum, etc.), Schizochytrium has many advantages such as fast growth and high DHA content, and is one ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/00C11B1/10
CPCC11B1/00C11B1/04C11B1/104
Inventor 窦光朋霍文严黄磊黎丽魏巍邵先豹
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com