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Extraction process of coriolus versicolor polysaccharide

A polysaccharide and extraction technology of Yunzhi, which is applied in the field of extracting effective components of Yunzhi, can solve the problems of affecting biological activity, high cost, and low extraction rate of polysaccharides, and achieve the goal of increasing biological activity, easy absorption and utilization, and simple process Effect

Inactive Publication Date: 2015-03-04
WEIHAI BAIHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method takes a long time, the extraction rate of polysaccharides is low, and the cost is high
In particular, the content of macromolecular protein in Yunzhi extract is high, because Yunzhi polysaccharide is a glucan containing protein, and macromolecular protein will affect its solubility and absorption rate, and affect its biological activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: a kind of Yunzhi polysaccharide extraction process, its specific steps are:

[0020] 1) Weigh 200g of processed versicolor versicolor powder, add 4000ml of water, soak for 90min, reflux in a constant temperature water bath at 50°C, and extract twice.

[0021] 2) The solution obtained in step 1) was centrifugally filtered to obtain the supernatant, and then absorbed by a macroporous resin to remove the color, and left to stand for 24 hours.

[0022] 3) Add cellulase and protease to the supernatant obtained in step 2), wherein the addition of cellulase with an enzyme concentration of 1.5% is 5.5% of the supernatant, and the addition of protease with an enzyme concentration of 1.5% is 3% of the supernatant was adjusted to pH 6.5, the temperature was 50°C, the time was 90 minutes, concentrated under reduced pressure to 70% of the original volume, and the supernatant was collected by suction filtration.

[0023] 4) The supernatant from step 3) was washed with ...

Embodiment 2

[0025] Embodiment 2: a kind of Yunzhi polysaccharide extraction process, its specific steps are:

[0026] 1) Weigh 100g of processed Yunzhi powder, add 2000ml of water, soak for 60min, reflux in a constant temperature water bath at 55°C, and extract twice.

[0027] 2) The solution obtained in step 1) was centrifugally filtered to obtain the supernatant, and then absorbed by a macroporous resin to remove the color, and left to stand for 24 hours.

[0028] 3) Add cellulase and protease to the supernatant obtained in step 2), wherein the amount of cellulase with an enzyme concentration of 1.5% is 4% of the supernatant, and the amount of protease with an enzyme concentration of 1.5% is 4% of the supernatant was adjusted to pH 6.5, the temperature was 45°C, the time was 60 minutes, concentrated under reduced pressure to 70% of the original volume, and the supernatant was collected by suction filtration.

[0029] 4) The supernatant from step 3) was washed with four times the volume...

Embodiment 3

[0030] Embodiment 3: a kind of Yunzhi polysaccharide extraction process, its specific steps are:

[0031] 1) Weigh 200g of the treated Yunzhi powder, add 5000ml of water, soak for 80min, reflux in a constant temperature water bath at 60°C, and extract twice.

[0032] 2) The solution obtained in step 1) was centrifugally filtered to obtain the supernatant, and then absorbed by a macroporous resin to remove the color, and left to stand for 24 hours.

[0033] 3) Add cellulase and protease to the supernatant obtained in step 2), wherein the addition of cellulase with an enzyme concentration of 1.5% is 6% of the supernatant, and the addition of protease with an enzyme concentration of 1.5% is 2% of the supernatant was adjusted to pH 6.5, the temperature was 55°C, the time was 80 min, concentrated under reduced pressure to 70% of the original volume, and the supernatant was collected by suction filtration.

[0034] 4) The supernatant from step 3) was washed with four times the volu...

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PUM

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Abstract

The invention relates to an extraction process of coriolus versicolor polysaccharide. The method comprises the following steps: extracting water-soluble polysaccharide from coriolus versicolor; after decolorization, adding cellulase and neutral protease, wherein the addition amount of the cellulase of which the enzyme concentration is 1.5 percent is 4-6 percent of that of a supernatant, the addition amount of the protease of which the enzyme concentration is 1.5 percent is 2-4 percent of that of the supernatant, the pH is adjusted as 6.5, the temperature is 45-55 DEG C, and the time is 60-90min; concentrating under reduced pressure until the volume is 70 percent of the original volume, and performing suction filtration to obtain clear liquid; washing the clear liquid by using absolute ethyl alcohol, and precipitating and drying the clear liquid to obtain a coriolus versicolor extract, wherein the content of the coriolus versicolor polysaccharide is 32-45 percent. The coriolus versicolor extract is obtained by double-enzyme enzymolysis fermentation, the process is simple, the cost is lower, and the extraction rate is high. Particularly, the cellulase is used for wall breaking treatment, and the protease is used for protein removal treatment, so that the defects of low solubility and the like caused by macromolecule protein are avoided, the biological activity of the coriolus versicolor polysaccharide is improved, and the coriolus versicolor polysaccharide can be better absorbed and utilized by a human body.

Description

technical field [0001] The invention relates to a method for extracting effective components of versicolor versicolor, in particular to an extraction process of versicolor versicolor polysaccharide. Background technique [0002] Yunzhi polysaccharide is a polysaccharide extracted from the basidiomycete versicolor versicolor, and can also be isolated from artificially cultivated mycelium. Has a variety of biological activities. Studies have shown that Yunzhi polysaccharide has immune regulation function, is a good immune enhancer, can enhance immune cell function and recognition ability, and increase the amount of IgM; Yunzhi polysaccharide also has liver protection effect, can significantly reduce serum transaminase, It has obvious repairing effect on liver tissue lesions and liver necrosis. Related polysaccharide preparations have been widely used clinically. The traditional method for extracting polysaccharides from Yunzhi includes a hot water extraction method, in whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王丽娜蔡传文张伟
Owner WEIHAI BAIHE BIOTECH
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