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Biological film inhibiting peptide and application thereof

A biofilm-inhibiting peptide and biofilm technology, applied in the field of biomedicine, can solve problems such as clinical hazards, lack of prevention and treatment methods for Staphylococcus epidermidis infection, and prolonged healing

Inactive Publication Date: 2015-03-04
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different from planktonic bacteria, BF cells have strict chemical signal transduction and exchange information, and establish a dense three-dimensional space structure, which can evade the attack of the host immune system and develop resistance to antibiotics, which is often manifested clinically Prolonged healing, causing great clinical harm
However, there is currently no effective prevention and treatment method for Staphylococcus epidermidis infection

Method used

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  • Biological film inhibiting peptide and application thereof
  • Biological film inhibiting peptide and application thereof
  • Biological film inhibiting peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, synthetic biomembrane inhibitory peptide

[0020] The biomembrane inhibitory peptide (hereinafter referred to as PT-8 polypeptide) contains 8 amino acid residues, and its sequence is: Pro Tyr Gly Gly Phe Ser Phe Thr (PYGGFSFT) (SEQ ID NO.1), using artificial standard solid-phase synthesis scheme, It was carried out on a solid-phase peptide synthesizer, the solid-phase support agent was resin, and the mobile phase was dimethylformamide / dichloromethane (1:1). The polypeptide shown in SEQ ID NO.1 is synthesized through multi-step reactions. Synthesized peptides using LC6000 high performance liquid chromatography, C 18 Column (Kromasil 20×250mm C 18 120A) for purification, the product peaks were collected to obtain a PT-8 polypeptide with a purity of 96.8%, vacuum-dried, weighed, divided into 20 mg / bottles, and stored at -20°C for future use. Then mass spectrometry was carried out, and the molecular weight was 874.4Da. The results showed that ESI-MS m / z: 87...

Embodiment 2

[0021] Embodiment 2, PT-8 polypeptide antibacterial test

[0022] (1) Determination of minimum inhibitory concentration (MIC) of PT-8 polypeptide against Staphylococcus epidermidis ATCC35984 by microbroth dilution method

[0023] Inoculate Staphylococcus epidermidis ATCC35984 on a Columbia blood plate, culture at 37°C for 24 hours, pick a single colony in a conical flask filled with 10ml of MH broth, shake at 37°C for 24 hours, then collect the bacterial liquid and dilute it to 10 6 CFU / ml, inoculate the diluted bacterial solution in a 96-well plate, 100 μL per well, and then add the peptide synthesized in Example 1 to a final concentration of 256 μg / ml, 128 μg / ml, 64 μg / ml, and 32 μg / ml , 16 μg / ml, 8 μg / ml, 4 μg / ml, 2 μg / ml and 1 μg / ml, and control the final volume to 200 μL, MH broth was used as blank control, and then placed at 37 ° C, 5% CO 2 Cultivate under conditions for 24 hours, observe with the naked eye, take the lowest drug concentration that inhibits bacterial gr...

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Abstract

The invention discloses a biological film inhibiting peptide and application thereof. The amino acid sequence of the biological film inhibiting peptide is as shown in SEQ ID NO.1. The biological film inhibiting peptide disclosed by the invention is simple in preparation method, can be synthesized through a conventional solid-phase synthesis method, is capable of inhibiting the formation and surface adhesion of a staphylococcus epidermidis biological film after being purified, can be jointly applied to inhibit drug-resistant staphylococcus epidermidis with other antibacterial drugs, and has great significance on clinically treating staphylococcus epidermidis infection.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a biomembrane inhibiting peptide, and also relates to the application of the biomembrane inhibiting peptide. Background technique [0002] Pathogenic bacteria represented by Staphylococcus epidermidis often adhere to the surface of implanted or invasive medical devices, including catheters, prostheses, artificial heart valves, artificial joints, etc., and form bacterial biofilms (BF ). Different from planktonic bacteria, BF cells have strict chemical signal transduction and exchange information, and establish a dense three-dimensional space structure, which can evade the attack of the host immune system and develop resistance to antibiotics, which is often manifested clinically Delayed healing has caused great clinical harm. However, there is currently no effective prevention and treatment method for Staphylococcus epidermidis infection. Therefore, there is an urgent nee...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P31/04
Inventor 陈勇川孙凤军欧阳净熊丽蓉冯伟
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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