A kind of autumn eggplant leaf total flavonoid nanoemulsion and its gel for whitening and freckle removal
A technology for whitening and removing freckles and autumn eggplant leaves, which is applied in the field of total flavonoid nanoemulsion of autumn eggplant leaves and its gel, can solve the problems of difficult nanoemulsion, poor solubility in water phase and oil phase, etc., and achieves inhibition of melanin production and inhibition of tyrosine. Acidase activity, the effect of improving curative effect
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Embodiment 1
[0050] Embodiment 1: prepare the high-purity candela leaf total flavonoids used in the present invention
[0051] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 60% as the extraction agent, the ratio of solid to liquid is 1: 15 (mass volume ratio), the extraction temperature is 80 ℃, the extraction time is 0.5 h, and the 2 times.
[0052] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD722 macroporous adsorption resin. The specific purification process is: the concentration of total flavonoids in the sample is 5.0mg / mL, and the flow rate of the sample is 3BV / h. The sample loading volume is 6.5 BV resin column volume, the adsorption time is 30 min, the impurities are washed with 3.5 BV water, the eluent is ethanol with a volume fraction of 50%, the elution flow rate is 3BV / h, and the amount of eluent is 4 BV resin column volume, collected the et...
Embodiment 2
[0053] Embodiment 2: Preparation of high-purity candela leaf total flavonoids used in the present invention
[0054] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 70% as the extractant, the ratio of solid to liquid is 1: 15 (mass volume ratio), the extraction temperature is 70°C, the extraction time is 0.5 h, and the extraction 2 times.
[0055] (2) Separation and purification: the total flavonoids of candela leaves obtained in step (1) were purified with HPD100 macroporous adsorption resin. The loading volume is 6BV resin column volume, the adsorption time is 30 min, and the impurity is washed with 4BV water, the eluent is ethanol with a volume fraction of 80%, the elution flow rate is 6BV / h, and the eluent dosage is 4 BV resin column volume , collected the ethanol liquid, recovered the solvent under reduced pressure, vacuum dried, and pulverized to obtain 93.2 g of total flavonoid powder of yellow-br...
Embodiment 3
[0056] Embodiment 3: Preparation of high-purity candela leaf total flavonoids used in the present invention
[0057] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 65% as the extractant, the ratio of solid to liquid is 1: 15 (mass volume ratio), the extraction temperature is 78°C, the extraction time is 0.5 h, and the extraction 2 times.
[0058] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD826 macroporous adsorption resin. The specific purification process is: the concentration of total flavonoids in the sample is 4.0mg / mL, and the flow rate of the sample is 3BV / h. The sample loading volume is 7BV resin column volume, the adsorption time is 30 min, the impurity is removed by washing with 3BV water, the eluent is ethanol with a volume fraction of 35%, the elution flow rate is 3BV / h, and the eluent dosage is 4 BV resin column volume , collect...
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