Microecological preparation used in prawn feeds and application thereof
A technology of micro-ecological preparations and prawn feed, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of reduced immunity of farmed animals, enhanced drug resistance of pathogens, etc., and achieve the goal of enhancing immunity, inhibiting pathogenic bacteria, and promoting growth Effect
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Embodiment 1
[0019] The preparation of embodiment 1 probiotics feed
[0020] The probiotics described in this embodiment for the prawn feed include: Bacillus subtilis 4 × 10 9 CFU / g, Bacillus licheniformis 1×10 9 CFU / g, honeysuckle extract (10:1), astragalus polysaccharide (60%).
[0021] The application of the microecological preparation of the present invention in the preparation of prawn feed: the added amount of the probiotic is 1‰ of the total weight of the feed. The detection of Bacillus subtilis after feed pelleting was 2.4×10 8 CFU / g, Bacillus licheniformis 3.8×10 7 CFU / g, extract content not detected.
Embodiment 2
[0022] Embodiment 2, microecological preparation feed prawn application test
[0023] The volume of the experimental aquarium was 60L, and it was continuously inflated during the breeding period. The experiment was divided into two groups, the control group (basic feed) and the test group (probiotic feed), with 5 repetitions in each group. Five vannamei shrimp were cultured in each aquarium. Before the test, 50 healthy Penaeus vannamei with similar body weight and body length were selected, with an average weight of (8.6±0.1) g, and were randomly divided into 10 aquariums. The test started after 1 week of domestication, and the feeding cycle was 40 days. Weighing after the test; blood collection to measure serum superoxide dismutase (SOD), alkaline phosphatase (AKP), acid phosphatase (ACP) activities; intestinal samples were taken to determine protease and amylase; kits were purchased from Nanjing Jiancheng Institute of Bioengineering.
[0024] Table 1 Relative weight gain ...
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