Bletilla striata large-scale high-yield cultivation method
A cultivation method and technology of bletilla striata, applied in the field of Chinese herbal medicine planting, can solve the problems of weakened medicinal value of bletilla striata, low reproduction efficiency, and slow economic benefits, so as to speed up photosynthesis and metabolism, reduce investment risks, and achieve economic benefits fast effect
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Embodiment 1
[0018] The concrete steps of this embodiment are as follows:
[0019] ① Seedling propagation: seedling propagation adopts the cultivation method of tissue culture propagation,
[0020] a. Seed selection: select wild bletilla striata plants that are growing vigorously, and use artificial assistance to carry out cross-pollination or pollination between flowers of different plants during the flowering period. After 4 to 6 months, collect mature and full capsules as exogenous seeds for tissue culture. Implant;
[0021] b. Seedling cultivation: add 0.5% volume ratio of EM original dew to the pre-prepared tissue culture solution, then soak the seeds inside the capsule in povidone iodine for 3-4 minutes, rinse with sterile water for 2-4 times Blot the water on the surface with sterile paper, and finally inoculate the sterilized seeds into the tissue culture medium, adjust the temperature, humidity and light reasonably, and germinate new shoots within 15 days;
[0022] ② Seedling ha...
Embodiment 2
[0030] The concrete steps of this embodiment are as follows:
[0031] ① Seedling propagation: seedling propagation adopts the cultivation method of tissue culture propagation,
[0032] a. Seed selection: select wild bletilla striata plants that are growing vigorously, and use artificial assistance to carry out cross-pollination or pollination between flowers of different plants during the flowering period. After 4 to 6 months, collect mature and full capsules as exogenous seeds for tissue culture. Implant;
[0033] b. Seedling cultivation: Add 1.3% volume ratio of EM original dew to the pre-prepared tissue culture solution, then soak the seeds inside the capsule in povidone iodine for 3 to 4 minutes, rinse with sterile water for 2 to 4 times Blot the water on the surface with sterile paper, and finally inoculate the sterilized seeds into the tissue culture medium, adjust the temperature, humidity and light reasonably, and germinate new shoots within 12 days;
[0034] ② Seedl...
Embodiment 3
[0042] The concrete steps of this embodiment are as follows:
[0043] ① Seedling propagation: seedling propagation adopts the cultivation method of tissue culture propagation,
[0044] a. Seed selection: select wild bletilla striata plants that are growing vigorously, and use artificial assistance to carry out cross-pollination or pollination between flowers of different plants during the flowering period. After 4 to 6 months, collect mature and full capsules as exogenous seeds for tissue culture. Implant;
[0045]b. Seedling cultivation: add 2% EM original dew to the pre-prepared tissue culture solution, then soak the seeds in the pod iodine for 3 to 4 minutes, rinse with sterile water for 2 to 4 times Blot the water on the surface with sterile paper, and finally inoculate the sterilized seeds into the tissue culture medium, adjust the temperature, humidity and light reasonably, and new shoots will germinate in 10 to 15 days;
[0046] ② Seedling hardening: Remove the seedli...
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