Calf spleen extractive and application thereof to idiopathic thrombocytopenic purpura treatment

An extract, calf technology, applied in calf spleen extract, preparation and treatment of primary immune thrombocytopenia, primary immune thrombocytopenia (ITP) treatment field, to achieve increased polypeptide and ribose content, relieve pain , the effect of reducing patient burden

Active Publication Date: 2015-02-18
JILIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing calf spleen extract production process has a low extraction rate, which directly affects the yield of the product, and the product quality is difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 500g of the spleen (within 24 hours after birth) of a dairy cow, wash it with 0.9% sodium chloride solution and soak it for 12 hours to remove blood and rinse; add 1 times the amount of normal saline according to the weight of the calf spleen The particle size is 2 ~ 8μm; thaw the homogenate at 40°C after deep freezing, repeat 5 times; add 0.17g papain, 0.5g cathepsin B, 0.83g 5'-phosphodiesterase, stir well; take out the homogenate and put Put it in a water bath below 90°C and raise the temperature to 75°C; keep the homogenate solution at 75°C for 2.5h and then cool it down to 20°C; Centrifuge for 15 minutes to collect the supernatant; adjust the pH of the extract to 7.0-7.2; use a 5000 Dalton membrane for ultrafiltration; 0.20 μm filter cartridge for sterile filtration to obtain the extract.

Embodiment 2

[0023] Take 500g of the spleen (within 24 hours after birth) of a dairy cow, wash it with 0.9% sodium chloride solution and soak it for 12 hours to remove the blood and rinse it; The particle size is 2-8μm; thaw the homogenate after deep freezing at 40°C, and repeat 10 times; 1.17g papain, 2.43g cathepsin B, 3.8g 5'-phosphodiesterase, stir well; take out the homogenate and put it in Warm up to 75°C in a water bath below 90°C; heat the homogenate solution at 75°C for 2 hours and then quickly cool down to 20°C; centrifuge the above solution at a speed of 3000-4000 rpm at a temperature of 0-10°C and centrifuge for 15- After 30 minutes, collect the supernatant; adjust the pH of the extract to 7.0-7.2; use a 5000 Dalton membrane for ultrafiltration; and filter it with a 0.20 μm filter element to obtain the extract.

Embodiment 3

[0025] Take 500g of cow spleen (within 24 hours after birth), wash with 0.9% sodium chloride solution and soak for 12 hours to remove blood and rinse; add 1-1.2 times the amount of normal saline according to the weight of calf spleen to homogenate 3 times, The particle size of the colloidal mill is 2 ~ 8 μm; deep freeze the homogenate and thaw it at 40°C, repeat 10 times; 0.78g papain, 2.34g cathepsin B, 3.9g 5'-phosphodiesterase, stir well; take out the homogenate Put it in a water bath below 90°C and raise the temperature to 80°C; heat the homogenate solution at 80°C for 2.5 hours and then cool it down to 10°C; centrifuge the above solution at a speed of 3000-4000 rpm and a temperature of 0-10°C. Centrifuge for 15-30 minutes to collect the supernatant; adjust the pH of the extract to 7.0-7.2; use a 5000 Dalton membrane for ultrafiltration; 0.20μm filter cartridge for sterile filtration to obtain the extract.

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PUM

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Abstract

The invention provides a calf spleen extractive and application thereof to idiopathic thrombocytopenic purpura treatment. The preparation technology of the calf spleen extractive comprises the steps of preparation and freeze thawing of a homogenate, and pH adjustment and film filtration under the synergistic effect of combinative enzymes, as well as sterile filtering and filling. The extraction rate of polypeptide and ribose in the calf spleen extractive is greatly increased, and the glycopeptide ratio is set to be 80-87. The activity, capable of treating ITP, of the calf spleen extractive is verified through blood platelet counting and CD4<+>CD25<+>T proportion in all the immune organs, and the result shows that the calf spleen extractive can remarkably improve the blood platelet level by 50% than the original process, the hematopoietic function is improved and no toxic or side effects is generated.

Description

Technical field: [0001] The invention relates to a calf spleen extract extracted by a new process, and also provides its use in the treatment of primary immune thrombocytopenia (ITP), belonging to the field of biomedicine. technical background: [0002] At present, calf spleen extract has been widely used in the adjuvant treatment of tumors and related diseases caused by immune system disorders such as vitiligo, urticaria and allergic reactions. In addition, calf spleen extract is also used clinically as a treatment and adjuvant therapy for primary immune thrombocytopenia (ITP). [0003] Peptides, ribose, and transfer factors are generally extracted from biological tissues by physical extraction methods, such as freeze-thaw methods, homogenization methods, and membrane filtration methods, such as the method disclosed in the Chinese invention patent (CN201010257796.0). The existing calf spleen extract production process has a low extraction rate, which directly affects th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61P7/04A61P7/06A61P37/02
CPCA61K35/26
Inventor 滕利荣王迪贾东旭孟庆繁逯家辉周毓麟滕乐生谢晶程瑛琨王贞佐蔡广胜卢文倩
Owner JILIN UNIV
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