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Application of 4-chlorocinnamaldehyde thiosemicarbazide in the control of plant diseases

A technology of cinnamaldehyde thiosemicarbazone and cinnamaldehyde amino shrinkage, which is applied in the field of agricultural plant protection, can solve the problems of reduced efficacy of fungicides, and achieve good control effect, good systemic conduction activity, and safe emergence

Active Publication Date: 2016-06-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1970s, benzimidazoles, dicarboxamides and anilinopyrimidine fungicides have been used in the control of Botrytis cinerea, but with the widespread use of these agents, Botrytis cinerea have produced Different degrees of drug resistance greatly reduce the effectiveness of fungicides

Method used

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  • Application of 4-chlorocinnamaldehyde thiosemicarbazide in the control of plant diseases
  • Application of 4-chlorocinnamaldehyde thiosemicarbazide in the control of plant diseases
  • Application of 4-chlorocinnamaldehyde thiosemicarbazide in the control of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 14

[0020] The bacteriostatic activity of embodiment 14-chlorocinnamaldehyde thiosemicarbazone to wheat take-all and Rhizoctonia graminearum

[0021] 1. Materials and methods

[0022] 1.1 Test materials

[0023] 1.1.1 Tested strains

[0024] Wheat take-all strain (Gaeumammomyces graminisvar.tritici) XX1 (provided by China Agricultural University)

[0025] Wheat sheath blight strain (Rhizoctonia acerealis) RC1 (provided by China Agricultural University)

[0026] 1.1.2 Drugs to be tested

[0027] 4-chlorocinnamaldehyde thiosemicarbazone (a yellow powder with a purity of 97%, provided by the College of Science, China Agricultural University)

[0028] 1.1.3 Preparation of medium

[0029] Preparation of Potato Dextrose Medium (PDA): Weigh 200g of peeled potatoes and cut into small pieces, add 1000ml of water and boil for half an hour, filter out the potato pieces with gauze, add water to make up 1000ml, then add 18g of glucose and 15g of agar, heat the agar After it is completely...

Embodiment 24

[0045] Example 24- Field control effect of chlorocinnamaldehyde thiosemicarbazone on wheat take-all and wheat sheath blight

[0046] 1. Materials and methods

[0047] 1.1 Test materials

[0048] 1.1.1 Wheat varieties

[0049] Zhou Mai 22

[0050] 1.1.2 Drugs to be tested

[0051] Test agents: 5% 4-chlorocinnamaldehyde thiosemicarbazone suspension concentrate, 12.5% ​​total eclipse (silthiopham, Silthiopham) suspension concentrate, 3% difenoconazole suspension concentrate.

[0052] Wherein, the preparation method of 5% 4-chlorocinnamaldehyde thiosemicarbazone suspension concentrate is as follows: adopt wet grinding method, with 4% surfactant 0201B, 1% high-purity sodium bentonite A, 5% 4-chloro Cinnamaldehyde thiosemicarbazone, 0.4% warning color (basic rhodamine), 2% ethylene glycol and water are successively added to a sand mill and ground for 45 minutes, so that the particle size of the product reaches below 4.0 microns to obtain a suspending agent.

[0053] Among them,...

Embodiment 34

[0088] The control of embodiment 34-chlorocinnamaldehyde thiosemicarbazones to tomato gray mold

[0089] 1. Materials and methods

[0090] 1.1 Test materials

[0091] 1.1.1 Tested strains

[0092] Test strains: Botrytis cinerea strain FJ1-3 (provided by China Agricultural University)

[0093] 1.1.2 Drugs to be tested

[0094] 4-chlorocinnamaldehyde thiosemicarbazone (a yellow powder with a purity of 97%, provided by the College of Science, China Agricultural University)

[0095] 1.1.3 Preparation of medium

[0096] The preparation of potato dextrose medium (PDA) is the same as in Example 1.

[0097] 1.2 Test method

[0098] 1.2.1 The cultivation of pathogenic bacteria

[0099] Botrytis cinerea was inoculated on a PDA plate, and placed in an incubator at 18°C ​​for 4 days in the dark.

[0100] 1.2.2 Indoor toxicity test

[0101] Prepare the test agent with dimethyl sulfoxide to a concentration of 1×10 5 The μg / ml mother solution was stored in a refrigerator at 4°C, an...

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Abstract

The invention relates to a novel application of 4-chlorocinnamaldehyde thiosemicarbazone in plant disease control. 4-chlorocinnamaldehyde thiosemicarbazone is taken as an active ingredient of a novel bactericide, is processed into emulsifiable concentrate or a suspending agent and used for spray treatment or seed treatment, better intake transportation activity of the active ingredient is played, and the problems of single medicament, unstable control effect, high control cost and the like of wheat root disease control in the current production as well as serious drug resistance in vegetable gray mold control are effectively solved. The control effect of the novel bactericide on three plant diseases including gaeumammomyces graminis var. tritici, wheat sharp eyespot and vegetable gray mold are researched by means of methods of indoor biological test and greenhouse and field efficacy tests. The bactericide is used for guaranteeing safe emergence of seedlings and healthy and strong seedlings after wheat seed treatment, and has good control effects on root diseases including gaeumammomyces graminis var. tritici, wheat sharp eyespot and the like, a yield increase function, characteristics of safety, effectiveness, economic efficiency as well as wide market application prospect.

Description

technical field [0001] The invention relates to the field of agricultural plant protection, in particular to the application of 4-chlorocinnamaldehyde thiosemicarbazone in the prevention and treatment of plant diseases. Background technique [0002] Wheat take-all (Gaeumammomycesgraminisvar.tritici) is an important root disease of wheat, which mainly damages the root system and stem base of wheat. According to statistics, mildly diseased fields reduce production by 10-30%, and seriously reduce production by more than 50%, or even extinction, which is one of the devastating diseases in wheat production. At present, there is no wheat variety resistant to take-all in my country. Although cultivation measures such as crop rotation and stubble inversion have certain effects on the control of take-all, it is difficult to popularize and apply it on a large scale due to limited cultivated land area and poor operability. According to reports such as Wang Gang (Wang Gang, Yang Zhiwei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N47/34A01P3/00
Inventor 刘西莉王治文杨新玲凌云徐焱彭钦侯艳华
Owner CHINA AGRI UNIV
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