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Method for measuring content of ginsenoside in heart-benefiting preparation

A technology for Yixinshu preparation and ginsenosides, which is applied in the field of quality detection of traditional Chinese medicines, and can solve the problems such as the determination of the content of ginsenoside Rc components.

Active Publication Date: 2015-02-04
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Determination of Ginsenoside Rg in Yixinshu Capsules by HPLC 1 , Re, Rb 1 (Chinese Journal of Experimental Formulas, Du Jiaxin, September 2010, Volume 16, No. 11, Pages 88-90) discloses the determination of ginsenoside Rg in Yixinshu capsules by HPLC 1 , Re, Rb 1 However, the existing quality testing method of Yixinshu traditional Chinese medicine preparation does not measure the content of ginsenoside Rc. In order to further improve the quality testing standard of Yixinshu traditional Chinese medicine preparation to ensure its drug quality, And in order to reduce the error caused by separate and multiple determinations of different ginsenoside components, if the content of multiple components can be determined at one time, it can not only save time and cost, but also make the test results under the same system conditions more stable and reliable. Therefore the applicant set out to study the simultaneous determination of ginsenoside Rg in Yixinshu preparations. 1 , Re, Rb 1 , Rc four-component content method

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  • Method for measuring content of ginsenoside in heart-benefiting preparation

Examples

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Effect test

Embodiment 1

[0088] Example 1 of the present invention: Ginsenoside Rg in a Yixinshu preparation 1 , Re, Rb 1 , the assay method of Rc, the steps are as follows:

[0089] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.02% phosphoric acid aqueous solution is used as mobile phase B, gradient elution, elution condition: 0 ~5min: A:B=25:75; 5~35min: A:B=25~35:75~65; 35~70min: A:B=35~50:65~50; 70~80min: A:B =50~25: 50~75; 80~110min: A:B=25:75; the detection wavelength is 203nm, the flow rate is 0.8mL / min, the column temperature is 25°C, and the number of theoretical plates is not low based on the peak of ginsenoside Re at 3000;

[0090] (2) Preparation of reference substance solution: get ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 , ginsenoside Rc reference substance amount, accurately weighed, respectively add methanol to make each 1mL containing ginsenoside Rg 1 0.1mg solutio...

Embodiment 2

[0093] Example 2 of the present invention: Ginsenoside Rg in a Yixinshu preparation 1 , Re, Rb 1 , the assay method of Rc, the steps are as follows:

[0094] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.2% phosphoric acid aqueous solution is used as mobile phase B, gradient elution, elution condition: 0 ~5min: A:B=25:75; 5~35min: A:B=25~35:75~65; 35~70min: A:B=35~50:65~50; 70~80min: A:B =50~25: 50~75; 80~110min: A:B=25:75; the detection wavelength is 203nm, the flow rate is 0.9mL / min, the column temperature is 30°C, the number of theoretical plates is not low based on the peak of ginsenoside Re at 3000;

[0095] (2) Preparation of reference substance solution: get ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 , ginsenoside Rc reference substance amount, accurately weighed, respectively add methanol to make each 1mL containing ginsenoside Rg 1 0.1mg solution, ev...

Embodiment 3

[0098] Example 3 of the present invention: Ginsenoside Rg in a Yixinshu preparation 1 , Re, Rb 1 , the assay method of Rc, the steps are as follows:

[0099] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use methanol as mobile phase A, 1% phosphoric acid aqueous solution as mobile phase B, gradient elution, elution condition: 0 ~5min: A:B=25:75; 5~35min: A:B=25~35:75~65; 35~70min: A:B=35~50:65~50; 70~80min: A:B =50~25: 50~75; 80~110min: A:B=25:75; the detection wavelength is 203nm, the flow rate is 1.0mL / min, the column temperature is 35°C, the number of theoretical plates is not low based on the peak of ginsenoside Re at 3000;

[0100] (2) Preparation of reference substance solution: get ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 , ginsenoside Rc reference substance amount, accurately weighed, respectively add methanol to make each 1mL containing ginsenoside Rg 1 0.1mg solution, every 1mL containing...

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Abstract

The invention discloses a method for measuring the content of ginsenoside in a heart-benefiting preparation. The method is a high performance liquid chromatography method for gradient elution by taking ginsenoside Rg1, Re, Rb1 and Rc contrasts as contrasts, methyl alcohol as a flowing phase and a 0.02-2% phosphoric acid aqueous solution as a flowing phase B. The method can be used for quickly and accurately measuring the contents of ginsenoside Rg1, Re, Rb1 and Rc components in the heart benefiting preparation in a high-repetitiveness and high-recycling-rate manner.

Description

technical field [0001] The invention relates to a method for determining the content of ginsenosides in Yixinshu preparations, belonging to the technical field of traditional Chinese medicine quality detection. Background technique [0002] Yixinshu traditional Chinese medicine preparation is composed of ginseng, astragalus, salvia miltiorrhiza, Ophiopogon japonicus, Schisandra chinensis, Chuanxiong, hawthorn and other seven herbs. It is widely used clinically to treat angina pectoris, myocardial ischemia, various types of arrhythmia, atrial premature beats, chest pain and angina pectoris of coronary heart disease. [0003] Yixinshu traditional Chinese medicine preparation contains ginseng, and ginsenoside is the main active ingredient of ginseng. There are about 40 kinds of GS monomers that are clearly known; the content in ginseng is about 4%. Numerous studies have shown that it has high anti-tumor activity, has no toxic side effects on normal cells, and has a synergistic...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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