Compounds with antitumor activity, and preparation method and application thereof

Abstract

The invention discloses gynostemma pentaphyllum extract compounds (I) with inhibiting actions on human liver cancer HepG2 cells and a preparation method thereof, and application of the compounds in medicine, belonging to the field of phytochemistry. The preparation method of the compounds disclosed as Formula (I) comprises the following steps: carrying out high-temperature high-pressure treatment on whole gynostemma pentaphyllum, extracting with ethanol under reflux, and concentrating the extracting solution under reduced pressure to obtain an extractum; adding distilled water into the extractum to form a suspensoid, and extracting sequentially with dichloromethane and ethyl acetate; concentrating under reduced pressure to obtain an ethyl acetate extract; passing the extract through an HP20 resin, eluting sequentially with 20%, 50% and 70% ethanol, and concentrating the 70% ethanol eluate under reduced pressure to obtain an elution product; and treating the elution product with silica gel column chromatography, eluting with dichloromethane / methanol in a ratio of 20:1-10:1 (v / v), sectionally collecting the eluates to obtain 6 components, and eluting the component 3 with acetonitrile / water in a ratio of 53:47 (v / v) at the flow rate of 3 mL / minute in a semipreparative chromatographic column (250*10mm, 5 mu m). The pharmacodynamical test indicates that the compounds disclosed as Formula (I) have obvious activities for inhibiting cancer cell proliferation.

Description

technical field [0001] The present invention relates to a ketone compound, in particular to a saponin compound with antitumor activity extracted from plant Gynostemma pentaphyllum; the present invention also relates to a preparation method of the saponin compound and the use of the compound in medicine The application belongs to the field of phytochemistry. Background technique [0002] Gynostemma is the whole herb of Gynostemma Gynostemma pentaphyllum (Thunb.) Makino of Cucurbitaceae. At present, there are many series of products such as Gynostemma pentaphyllum (Thunb.) Makino. Modern pharmacology shows that Gynostemma pentaphyllum has anti-cancer, anti-oxidation, hypoglycemic, hypolipidemic, and immune-enhancing effects. [0003] Some saponin compounds with strong activity in natural plants have limited sources and low content in plants. Traditional separation methods not only require a large amount of medicinal resources, but also have low yields. Therefore, how to incr...

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Problems solved by technology

[0003] Some saponin compounds with strong activity in natural plants have limited sources and low content in plants. Traditional separation methods not only require a large amount of medicinal resources, but also have low yields.

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