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Living spore content high throughput detection method based on spectrophotometry method

A technology of spectrophotometry and detection method, which is applied in the field of modern microbiology technology and fermentation engineering, can solve the problems of high cost, heavy workload, and long cycle, and achieve the effect of low cost, reduced workload, and less consumption of reagents

Active Publication Date: 2015-01-28
SHENZHEN UNIV
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Problems solved by technology

[0007] In order to overcome the problems of large workload, long period and high cost in the conventional method commonly used to detect the content of live spores, the present invention provides a high-throughput detection method based on non-cultivation and microplate reader light absorption detection

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  • Living spore content high throughput detection method based on spectrophotometry method
  • Living spore content high throughput detection method based on spectrophotometry method
  • Living spore content high throughput detection method based on spectrophotometry method

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Embodiment Construction

[0036] The present invention will be described more specifically through the following examples, and the contents mentioned in the examples are not intended to limit the present invention.

[0037] The impact of yeast powder concentration on the yield of Bacillus sp.H4 viable spores, compared the results obtained by the conventional colony counting method and the method of the present invention, to verify the feasibility of the spectrophotometric method.

[0038] The strain Bacillus sp.H4 to be tested is an alkaliphilic Bacillus, and the strain Bacillus subtilis ATCC6051 with a neutral pH was selected as the source of non-germinating spores. Inoculate Bacillus sp.H4 on the alkaline whole yeast solid medium at 8 mm intervals and culture for 7-10 days. Inoculate Bacillus subtilis ATCC6051 on whole yeast solid medium at 8 mm intervals and culture for 7-10 days. Pick the colony of Bacillus subtilis ATCC6051 on the solid medium with a sterilized toothpick into sterilized deionized...

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Abstract

The invention discloses a living spore content high throughput detection method based on spectrophotometry method, once spore encounters a suitable environment, the spore may germinate rapidly, light refraction property of the spore converted from a dormant state into a vegetative growth state is very strong, and in the process of germination, light absorption decrease is caused by water absorption swelling and light refraction property disappearance of the multilayer structure. The light absorption decrease as the characterization of spore germination activity is used to evaluate the spore reproduction vitality, can greatly simplify the detection process of living spore content, and overcomes the problems of detection workload and long cycle and high cost of a traditional method.

Description

technical field [0001] The invention relates to a high-throughput detection method for detecting live spore content based on spectrophotometry, and belongs to the technical fields of modern microbiology technology and fermentation engineering. Background technique [0002] Spores are round or oval stress-resistant endogenous dormant bodies formed in cells by certain bacteria in the later stages of growth and development. Spores are highly resistant to heat, ultraviolet light, electromagnetic radiation and certain chemicals, and can tolerate a variety of adverse and even extreme environments. [0003] Bacillus is an important microbial group closely related to humans. Bacillus contains many strains with special functions, which have a wide range of application value in scientific fields such as industry, agriculture, and medicine, and have attracted more and more attention and research. Bacillus is widely used in agriculture. Many species represented by Bacillus cereus can ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 张金龙邓旭邢锋卢靖坤柳秋月张妙君靳帆
Owner SHENZHEN UNIV
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