Preparation and application of sika deer spleen extract having function of increasing white blood cells
A technology for increasing white blood cells and sika deer, applied in the direction of extracellular fluid diseases, medical preparations containing active ingredients, applications, etc., to achieve the effect of increasing content and activity
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Embodiment 1
[0020] . Weigh 250g of sika deer spleen and remove the surface connective tissue;
[0021] . Add 750ml of normal saline and grind for 5 minutes with a colloid mill;
[0022] .Heat the deer spleen suspension to 50°C, adjust the pH value to 5, add 0.25g neutral protease, 0.75g trypsin and 1.5g 5'-phosphodiesterase at the same time to incubate for 2 hours, after the incubation is completed, under 4000g centrifugal force Carry out centrifugation, obtain supernatant liquid I;
[0023] .The deer spleen precipitate obtained in the previous step continued to be added with an equal mass of normal saline, and the temperature was raised to 60°C. At the same time, 0.12g of neutral protease, 0.48g of papain and 0.6g of 5'-phosphodiesterase were added to incubate for 3 hours, and then in Centrifuge under 4000g centrifugal force for 10min to obtain supernatant II liquid;
[0024] e. Mix the supernatant liquid I and liquid II, then perform ultrafiltration with a 10KD ultrafiltration...
Embodiment 2
[0026] . Weigh 250g of sika deer spleen and remove the surface connective tissue;
[0027] . Add 1200ml of normal saline, and grind for 5 minutes with a colloid mill;
[0028] .Heat the deer spleen suspension to 55°C, adjust the pH value to 5, add 0.51g neutral protease, 1.48g trypsin and 3.1g 5'-phosphodiesterase at the same time and keep it warm for 2 hours. centrifuged at a lower temperature to obtain the supernatant liquid I;
[0029] .The deer spleen precipitate obtained in the previous step was continued to be added with an equal mass of normal saline, and the temperature was raised to 60°C. At the same time, 0.22g of neutral protease, 0.78g of papain and 1.05g of 5'-phosphodiesterase were added to incubate for 1h, and then in Centrifuge under 4000g centrifugal force for 10min to obtain supernatant II liquid;
[0030] e. Mix the supernatant liquid I and liquid II, then perform ultrafiltration with a 10KD ultrafiltration membrane, and spray-dry the ultrafiltrate...
Embodiment 3
[0032] . Weigh 250g of sika deer spleen and remove the surface connective tissue;
[0033] . Add 1400ml of normal saline and grind for 4 minutes with a colloid mill;
[0034] .Heat the deer spleen suspension to 50°C, adjust the pH value to 5, add 0.54g neutral protease, 1.62g trypsin and 3.52g 5'-phosphodiesterase at the same time and incubate for 1h. After the incubation, under 4000g centrifugal force Carry out centrifugation, obtain supernatant liquid I;
[0035] .The deer spleen precipitate obtained in the previous step was continued to be added with an equal mass of normal saline, and the temperature was raised to 60°C. At the same time, 0.28g of neutral protease, 0.89g of papain and 1.45g of 5'-phosphodiesterase were added to incubate for 2h, and then in Centrifuge under 4000g centrifugal force for 10min to obtain supernatant II liquid;
[0036] e. Mix the supernatant liquid I and liquid II, then perform ultrafiltration with a 10KD ultrafiltration membrane, and ...
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