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Rapid propagation method for culturing suspension cells of swertia pseudochinensis

A technology of swede swede and suspended cells, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as the lack of research on cultivation methods, and achieves advantages of large-scale planting, short cycle and low pollution. Effect

Inactive Publication Date: 2015-01-28
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Indications for jaundice hepatitis, dysentery, stomach pain, indigestion, toothache, red eyes, headache, mouth sores; external use to treat sores, there is no research on its cultivation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the Swertia pilosa seeds, wash them, soak them in washing powder for 25 minutes, rinse them with running water for 2.5 hours, treat them with 75% alcohol on an ultra-clean workbench for 10 minutes, treat them with 0.2% mercury chloride for 23 minutes, rinse them with sterile water 5 times, and disinfect them The treated seeds were placed and inserted into the H+NAA0.01mg / L+6-BA0.3mg / L+ZT0.8mg / L medium in the light incubator for callus induction, with 7g / L agar added, 30g / L L sucrose, temperature 25°C, the induced callus was inserted into medium H+ZT0.5mg / L+KT0.5mg / L for callus proliferation culture, additional 7g / L agar, 30g / L sucrose, light 3500lx, light time 12h / d, temperature 25°C, callus with emerald green color and uniform structure after proliferation, inoculate into H+ZT0.3mg / L+KT0.3mg / L medium for suspension shaking culture, add 30g / L sucrose, temperature 25°C, light 3500lx, light time 12h / d, amplitude 3cm, vibration frequency 100r / min, suspension cell gro...

Embodiment 2

[0011] Take the Swertia pilosa seeds, wash them, soak them in washing powder for 25 minutes, rinse them with running water for 2.5 hours, treat them with 75% alcohol on an ultra-clean workbench for 10 minutes, treat them with 0.2% mercury chloride for 23 minutes, rinse them with sterile water 5 times, and disinfect them The treated seeds were placed and inserted into the H+NAA0.01mg / L+6-BA0.3mg / L+ZT0.8mg / L medium in the light incubator for callus induction, with 7g / L agar added, 30g / L L sucrose, temperature 25°C, the induced callus was inserted into medium H+ZT0.5mg / L+KT0.5mg / L for callus proliferation culture, additional 7g / L agar, 30g / L sucrose, light 3500lx, light time 12h / d, temperature 25°C, callus with emerald green color and uniform structure after proliferation, inoculated into H+ZT0.5mg / L+KT0.5mg / L medium for suspension shaking culture, add 30g / L sucrose, temperature 25°C, light 3500lx, light time 12h / d, amplitude 3cm, vibration frequency 100r / min, growth rate of sus...

Embodiment 3

[0013] Take the Swertia pilosa seeds, wash them, soak them in washing powder for 25 minutes, rinse them with running water for 2.5 hours, treat them with 75% alcohol on an ultra-clean workbench for 10 minutes, treat them with 0.2% mercury chloride for 23 minutes, rinse them with sterile water 5 times, and disinfect them The treated seeds were placed and inserted into the H+NAA0.01mg / L+6-BA0.3mg / L+ZT0.8mg / L medium in the light incubator for callus induction, with 7g / L agar added, 30g / L L sucrose, temperature 25°C, the induced callus was inserted into medium H+ZT0.5mg / L+KT0.5mg / L for callus proliferation culture, additional 7g / L agar, 30g / L sucrose, light 3500lx, light time 12h / d, temperature 25°C, callus with emerald green color and uniform structure after proliferation, inoculate into H+ZT0.3mg / L+KT0.5mg / L medium for suspension shaking culture, add 30g / L sucrose, temperature 25°C, light 3500lx, light time 12h / d, amplitude 3cm, vibration frequency 100r / min, suspension cell gro...

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Abstract

The invention discloses a rapid propagation method for culturing suspension cells of swertia pseudochinensis. The rapid propagation method comprises the following steps: obtaining sterile seedlings, inducing calli, propagating the calli, culturing suspension cells and the like. The swertia pseudochinensis prepared by the method disclosed by the invention is high in callus rate, short in production period and high in yield and lays a research foundation for industrial production.

Description

technical field [0001] The invention relates to a rapid propagation method for suspension cell culture of Swertia pilosa, belonging to the field of biotechnology. Background technique [0002] Swertia chinensis, Gentianaceae, Swertia pseudochinensis Hara , also known as: as medicine, purple flower. The medicine is an annual herb, mainly produced in China, North Korea, and Russia. It is widely seen in the northern provinces in China, and the whole plant can be used as medicine. Born in hillside meadows, meadows by valleys and streams, distributed in my country's Hebei, Gansu, Sichuan, Liaoning, Jilin, Shandong, Shanxi, Henan, Beijing, northern Yanshan, and Ordos. The whole plant contains alkaloids, gentianine, flavonoids, flavonoids, isovitexin, xanthones, gentianone, xanthones, methyl xanthones, methyl The base daisy leaf gentianone, the opposite leaf drug xanthonone and the trace amount of demethylated drug xanthonone. In addition, it still contains yellow gentianin, ye...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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