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Method for high-density culture of bacillus subtilis

A high-density cultivation technology of Bacillus subtilis, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of few reports on high-density cultivation of Bacillus subtilis fermentation technology, and improve product efficiency , increase yield, increase the effect of bacterial volume

Inactive Publication Date: 2015-01-21
ENG TECH INST CO LTD OF CNSIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic research on Bacillus subtilis mainly focuses on bacteria screening, antibacterial effect and enzyme production characteristics of Bacillus subtilis, while there are few reports on the fermentation technology of high-density culture Bacillus subtilis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0029] (1) Seed cultivation:

[0030] The medium composition used for seed activation is as follows: peptone 10g / L, beef powder 3g / L, sodium chloride 5g / L, glucose 1g / L.

[0031] The conditions for the activation culture are: shaker culture; pH value 7.5; temperature 30° C.; time 24 h; inoculum size 8% (v / v).

[0032] (2) Fermentation culture:

[0033] The activated Bacillus subtilis was inoculated into the fermentation medium for cultivation, and the fermentation volume was 30L.

[0034] The fermentation medium is composed of the following raw materials: glucose 10g / L, bean cake powder 15g / L, MnSO 4 0.3g / L, NaCl5g / L, K 2 HPO 4 0.3g / L, calcium carbonate 5g / L.

[0035] The conditions of fermentation culture are: natural pH; culture temperature 30°C; rotation speed 200r / min; oxygen flow rate 2m 3 / h; liquid volume 30L; inoculum volume 8% (v / v); culture time 48h.

[0036] At the end of the culture, the number of viable bacteria was 8.9×10 9 cfu / mL.

example 2

[0038] Bacillus subtilis is used as the fermentation strain, which is inoculated into the fermentation medium after being activated by the tertiary seeds for cultivation.

[0039] (1) Activation culture: peptone 10g / L, beef powder 3g / L, sodium chloride 5g / L, glucose 1g / L; the conditions of activation culture are: shaker culture; pH value 7.5; temperature 30°C; time 24h .

[0040] (2) Fermentation culture:

[0041] The activated Bacillus subtilis was inoculated into the fermentation medium for cultivation, and the fermentation volume was 300L.

[0042] The fermentation medium is composed of the following raw materials: glucose 10g / L, bean cake powder 15g / L, MnSO 4 0.3g / L, NaCl5g / L, K 2 HPO 4 0.3g / L, calcium carbonate 5g / L.

[0043] The conditions of fermentation culture are: natural pH; culture temperature 30°C; rotation speed 200r / min; oxygen flow rate 15m 3 / h; liquid volume 30L; inoculum volume 8% (v / v); culture time 48h.

[0044] At the end of the culture, the number...

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Abstract

The invention relates to a method for high-density culture of bacillus subtilis. The method comprises the steps of (1) carrying out three-level seed activation by regarding bacillus subtilis as fermentation bacteria, wherein a activation culture medium comprises the components: 10g / L of peptone, 3g / L of beef powder, 5g / L of sodium chloride and 1g / L of glucose; (2) inoculating activated bacteria to a fermentation culture medium for culture, wherein the fermentation culture medium comprises the components: 10g / L of glucose, 15g / L of soybean cake powder, 0.3g / L of MnSO4, 5g / L of NaCl, 0.3g / L of K2HPO4, 5g / L of calcium carbonate; fermenting the components at a temperature of 30 DEG C under a stirring rotating speed of 200r / min for 48h to obtain high-density cultured bacillus subtilis. By improvement of culture medium prescriptions and fermentation conditions, viable count of bacillus subtilis is more than 8.9*10<9>cfu / mL, necessary conditions are provided for industrial fermentation of bacillus subtilis, and rate of production can be increased greatly.

Description

technical field [0001] The invention belongs to the technical field of fungi culture, in particular to a method for high-density culture of Bacillus subtilis. Background technique [0002] Bacillus subtilis can produce extracellular enzymes during the reproduction process in the water body, which can decompose organic matter such as residual bait, excrement waste, animal and plant residues in the water body and sediment, and these organic matters are first decomposed into small molecules, such as higher fatty acids and polypeptides, etc., and then decompose into smaller molecular organic substances, such as monosaccharides, amino acids, lower fatty acids and cyclic hydrocarbons, etc., and finally decompose into carbon dioxide, nitrate, sulfate, etc., and finally reduce the BOD and COD in the water. The concentration of nitrogen, ammonia, sulfide and nitrite nitrogen is reduced, which can remove the sediment and reduce the eutrophication of the water body. In addition, variou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 张云泽辛乃宏李炳乾刘颖芬
Owner ENG TECH INST CO LTD OF CNSIC
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