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Preparation method of feather protein powder

A feather protein powder, feather meal technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of large amino acid damage in feather meal, potential safety hazards, complicated procedures, etc., and achieve high product purity, wide application, and technology. simple steps

Inactive Publication Date: 2015-01-21
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, this method has complex procedures and hidden safety hazards, and on the other hand, the amino acid in the feather meal is greatly damaged, and the crude protein content in the prepared feather protein powder reaches 90%, and the prepared feather protein powder can only be used as feed protein

Method used

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  • Preparation method of feather protein powder
  • Preparation method of feather protein powder
  • Preparation method of feather protein powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] one. Materials and methods

[0024] Material

[0025] Chicken feathers (obtained from poultry slaughterhouse), keratinase: 100000U / g (Jinan Nuoneng Bioengineering Co., Ltd.), neutral protease: 30000U / g (Jinan Nuoneng Bioengineering Co., Ltd.), 1mol / L NaOH solution, 1mol / LHCL solution, distilled water, Coomassie Brilliant Blue Reagent (weigh 100mg Coomassie Brilliant Blue G-250 and dissolve in 50ml 95% ethanol, add 100mL85% phosphoric acid, add distilled water to dilute to 1000mL.), protein standard solution (crystalline bovine serum albumin, pre-tested Micro-Kjeldahl method was used to determine protein nitrogen content, and a protein standard solution of 100 μg / mL was prepared according to its purity.), pepsin (the actual titer of protease is 1:3000).

[0026] Main equipment

[0027] main instrument

[0028] 18 250mL triangular flasks, pipette (10mL), test tube and test tube rack, volumetric flask (50mL), mortar, 100 mesh standard sieve, dropper, funnel, filter pa...

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Abstract

The invention relates to the technical field of animal feed processing, and particularly discloses a preparation method of feather protein powder. The preparation method of the feather protein powder takes chicken feathers as a raw material, and is characterized by comprising the steps of carrying out pretreatment on the chicken feathers, and grinding into feather powder; putting the feather powder into a container, and feeding distilled water into the container, wherein 0.015-0.0525g of keratinase or 0.050-0.175g of neutral protease is fed into 1g of feather powder; adjusting the pH value to be 6-8.5, and carrying out enzymolysis for 4-48 hours at the temperature of 35-60 DEG C; and filtering enzymolysis reaction liquid, and taking out the filtrate which is the product. The preparation method is simple in processing steps and less in cost and investment; the product is high in purity; the product can be processed into amino acid products and feed additive protein, thus being relatively wide in application and suitable for wide popularization and application.

Description

(1) Technical field [0001] The invention relates to the technical field of animal feed processing, in particular to a preparation method of feather protein powder. (2) Background technology [0002] At present, the environmental pollution caused by the slaughtering industry in our country is quite serious, mainly because a large amount of feathers, blood, internal organs, hoof shells and other products will be produced while slaughtering livestock and poultry to produce meat, which has caused great pollution to the environment. Taking 2010 as an example, my country's poultry slaughtering industry reached about 11 billion. According to the calculation that each poultry can produce 0.10g of feathers, the feather production is about 1.1 million tons, which is equivalent to about 900,000 tons of protein resources. [0003] On the other hand, in today's increasingly scarce protein resources in our country, it is very important to make full use of feather protein resources. H...

Claims

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Application Information

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IPC IPC(8): A23K1/10
Inventor 郝鲁江范秋苹孟凡进冯昱畅张涛
Owner QILU UNIV OF TECH
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