A coaxial positioning capillary electrophoresis-electrochemical integrated detection device

Abstract

The invention discloses a coaxial positioning capillary electrophoresis-electrochemical integration detection device and relates to the technical field of electrophoresis-electrochemistry. The device comprises a coaxial positioning mechanism, an electrophoresis cathode and a three-electrode system, wherein the three-electrode system consists of a micro disc electrode, an auxiliary electrode and a reference electrode; and according to the coaxial positioning mechanism, a micro disc electrode guide pipe and a capillary tube guide pipe are surrounded and clamped by three rigid glass rods, so that pore channels of the two guide pipes are coaxially aligned, and internal pore channels of the two guide pipes are introduction channels of the micro disc electrode for electrochemical detection and a capillary tube for electrophoretic separation. The introduction channels of the capillary tube and the micro disc electrode for electrochemical detection are fixedly adhered by utilizing the surrounding glass rods, so that high-precision coaxial positioning is carried out, end-column emitter wall type capillary electrophoresis-electrochemical detection of to-be-detected mixed components can be rapidly performed, and a result is accurate and reliable.

Description

[0001] Technical field: [0002] The invention relates to the technical field of electrophoresis-electrochemistry, in particular to a coaxially positioned capillary electrophoresis-electrochemistry integrated detection device. [0003] Background technique: [0004] Capillary electrophoresis is a new type of liquid phase separation technology that uses a high-voltage direct current electric field as the driving force and a quartz capillary as the separation channel to achieve efficient separation based on the difference in mobility (including distribution behavior) between components in the sample. The inner surface of the quartz capillary used is SiO 2 Hydrate to form silanol (Si-OH), when the tube is filled with operating fluid with pH>3, the silanol will dissociate to form Si-O - . Therefore, the inner wall of the electrophoresis capillary has a lot of negative charges; while the operating fluid in the tube is positively charged to maintain the electrical neutrality of ...

AI Technical Summary

Problems solved by technology

The device of this detection method is generally composed of a three-electrode system (working electrode, reference electrode, auxiliary electrode); the completion of the detection process depends on the manual three-dimensional adjustment instrument (f) to adjust the working surface ( a) Accurate alignment with the outlet end of the electrophoretic separation capillary with an inner diameter of less than 100 μm (d), the process is inefficient and lacks reliability

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