A method for improving the preservation effect of Arabidopsis thaliana seedlings
A technology for Arabidopsis thaliana and seedlings is applied in the field of improving the preservation effect of Arabidopsis thaliana seedlings to achieve the effects of changing the formation state, improving viscosity and thermal conductivity, and reducing damage
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Embodiment 1
[0055] 1) Acquisition of Arabidopsis seedlings: Disinfect Arabidopsis seeds with 70% ethanol for 15 seconds, rinse them with sterile water for 4 times, then disinfect them with an aqueous solution containing 20wt% NaClO and 0.01wt% Tween20 for 10 minutes, and rinse them with sterile water for 6 times ; Then the sterilized seeds were vernalized at 4°C for 48 hours, and placed on MS solid medium for cultivation. The cultivation conditions were 8 hours of light cultivation per day, and the light intensity was 150 μmol / m 2 , cultivated at a temperature of 25° C.; then cultivated in the dark for 16 h at a culture temperature of 20° C., and cultured the seeds for a total of 60 h to obtain Arabidopsis seedlings with a seedling age of 60 h.
[0056] 2) Divide the 60h Arabidopsis seedlings into the experimental group and the control group, and set up 3 parallel experiments in each group, put 50 seedlings into a 2mL cryovial containing 1mL loading solution, and treat them at room tempera...
Embodiment 2
[0069] This example is mainly to test and analyze the parameters of Arabidopsis thaliana seedlings at various stages, so as to demonstrate the regulatory effect of exogenous carbon nanotubes on the membrane damage and oxidative stress during the cryopreservation of Arabidopsis thaliana seedlings for 60 hours.
[0070] 1) Experimental material: the material in group 1 is: the seedling of Arabidopsis thaliana germinated according to the method described in Example 1 of the present invention for 60h
[0071] Materials in group 2 are: seedlings treated with vitrification solution in Example 1;
[0072] Group 3 is: the seedlings after thawing after vitrification cryopreservation according to the method in Example 1;
[0073] Group 4 is the seedling after washing with washing liquid in embodiment 1;
[0074] Group 5 is the seedlings after recovery culture treatment in Example 1.
[0075] 2) Test method:
[0076] 1. Determination of relative conductivity during cryopreservation of...
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