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A method for improving the preservation effect of Arabidopsis thaliana seedlings

A technology for Arabidopsis thaliana and seedlings is applied in the field of improving the preservation effect of Arabidopsis thaliana seedlings to achieve the effects of changing the formation state, improving viscosity and thermal conductivity, and reducing damage

Inactive Publication Date: 2016-04-06
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adding certain exogenous substances in the existing technology is beneficial to improve the survival rate of cryopreservation, but it is unknown which exogenous substances to choose for specific plants and tissues to improve their preservation effect

Method used

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  • A method for improving the preservation effect of Arabidopsis thaliana seedlings
  • A method for improving the preservation effect of Arabidopsis thaliana seedlings
  • A method for improving the preservation effect of Arabidopsis thaliana seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1) Acquisition of Arabidopsis seedlings: Disinfect Arabidopsis seeds with 70% ethanol for 15 seconds, rinse them with sterile water for 4 times, then disinfect them with an aqueous solution containing 20wt% NaClO and 0.01wt% Tween20 for 10 minutes, and rinse them with sterile water for 6 times ; Then the sterilized seeds were vernalized at 4°C for 48 hours, and placed on MS solid medium for cultivation. The cultivation conditions were 8 hours of light cultivation per day, and the light intensity was 150 μmol / m 2 , cultivated at a temperature of 25° C.; then cultivated in the dark for 16 h at a culture temperature of 20° C., and cultured the seeds for a total of 60 h to obtain Arabidopsis seedlings with a seedling age of 60 h.

[0056] 2) Divide the 60h Arabidopsis seedlings into the experimental group and the control group, and set up 3 parallel experiments in each group, put 50 seedlings into a 2mL cryovial containing 1mL loading solution, and treat them at room tempera...

Embodiment 2

[0069] This example is mainly to test and analyze the parameters of Arabidopsis thaliana seedlings at various stages, so as to demonstrate the regulatory effect of exogenous carbon nanotubes on the membrane damage and oxidative stress during the cryopreservation of Arabidopsis thaliana seedlings for 60 hours.

[0070] 1) Experimental material: the material in group 1 is: the seedling of Arabidopsis thaliana germinated according to the method described in Example 1 of the present invention for 60h

[0071] Materials in group 2 are: seedlings treated with vitrification solution in Example 1;

[0072] Group 3 is: the seedlings after thawing after vitrification cryopreservation according to the method in Example 1;

[0073] Group 4 is the seedling after washing with washing liquid in embodiment 1;

[0074] Group 5 is the seedlings after recovery culture treatment in Example 1.

[0075] 2) Test method:

[0076] 1. Determination of relative conductivity during cryopreservation of...

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Abstract

The invention discloses a method for improving the storage effect of arabidopsis thaliana seedlings. The arabidopsis thaliana seedlings are treated by adopting a vitrification solution containing a carbon nano material so that the storage effect is improved. The method specifically comprises the following steps: processing by adopting a loading solution, processing by adopting the vitrification solution and storing by adopting liquid nitrogen, wherein the vitrification solution contains 0.1-0.5g / L of carbon nano tubes. The method disclosed by the invention has an optimal and remarkable storage effect for the arabidopsis thaliana seedlings; vitrification ultralow temperature plant storage is promoted by adding the carbon nano tubes as an exogenous material.

Description

technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for improving the preservation effect of Arabidopsis thaliana seedlings. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the material and its vitrification solution solidify int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00A01H4/00
Inventor 陈冠群任丽张荻申晓辉
Owner SHANGHAI JIAOTONG UNIV
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