An electrochemical biosensor, its preparation method and use

A biosensor and electrochemical technology, applied in the field of biosensors, achieves the effects of low cost, high sensitivity and simple synthesis method

Active Publication Date: 2017-01-11
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the reported electrochemical biosensors based on molecularly imprinted polymer membranes are only used to detect one analyte—the template molecule
Few researched systems detect multiple target analytes

Method used

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  • An electrochemical biosensor, its preparation method and use
  • An electrochemical biosensor, its preparation method and use
  • An electrochemical biosensor, its preparation method and use

Examples

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Embodiment Construction

[0050] The present invention will be described in detail below according to the accompanying drawings, which is a preferred embodiment among various implementations of the present invention.

[0051] The preparation of the electrochemical sensor based on the folic acid imprinted polymer film and the steps of its detection of the folic acid receptor and mercury ions are as follows:

[0052] a. Prepare the purchased synthetic polymer film into a solution of specific concentration for later use, dissolve the purchased DNA sequence (aminated at one end) in Tris-HCl (pH7.4) buffer solution, and store it at low temperature for later use .

[0053] b. Polish the gold electrode with 0.3 and 0.5 μm aluminum powder in turn, and then put it into HNO 3 :H 2 O(v / v)=1:1 solution, ethanol solution, and ultrapure water were used for ultrasonic cleaning, and the ultrasonic cleaning time was 3 to 5 minutes respectively.

[0054] c. Using a clean bare gold electrode as a working electrode, us...

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Abstract

The invention relates to an electrochemical biosensor as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) preparing drugs for synthesizing a polymer membrane into a solution with a certain concentration for standby; (2) dissolving a DNA sequence in a Tris-HCl buffer solution for standby; (3) polishing a gold electrode, and then conducting ultrasonic cleaning; (4) taking the cleaned bare gold electrode as a working electrode, taking the polymer solution with certain concentrate of template molecules as an electrolyte solution, and utilizing an electric polymerization process to synthesize the polymer modified gold electrode; (5) soaking and cleaning the obtained polymer modified electrode, and removing the template molecules in the polymer to obtain the molecularly imprinted membrane modified gold electrode; (6) obtaining the electrochemical biosensor established based on the molecularly imprinted polymer membrane. According to the invention, the advantages of the electrochemical biosensor and the template molecule technology are combined, and the synthetic method is simple, and low in energy consumption and cost; the template molecules can be released through the utilization of the specific binding function between the target analyte and the template molecules namely folic acid or the dissection function of the excision enzyme III, so that purpose that the folic acid receptor and mercury ions are respectively detected can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to an electrochemical sensor assembled with a polymer film composed of template molecules and polymers, and uses the sensor to analyze and detect folic acid receptors and mercury ions. Background technique [0002] In the field of sensors, molecular imprinting technology has aroused extensive interest of researchers due to its high selective recognition properties, simple synthesis and high stability. Electrochemical biosensors have become one of the most promising analytical methods this year because of their low manufacturing cost, simple instrumentation, simple operation, and rapid response. Therefore, electrochemical biosensors based on molecularly imprinted polymer membranes, which combine the inherent advantages of the two, have great potential in the fields of bioanalysis, drug analysis, environmental monitoring and evaluation, and pesticide residue detection, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/38G01N27/327G01N27/26
Inventor 王广凤陈玲
Owner ANHUI NORMAL UNIV
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