Nucleic acid membrane strips and kits for Candida species identification and gene mutation detection
A technology for bacterial species identification and nucleic acid membrane strips, which can be used in microorganism-based methods, microorganism determination/inspection, biochemical equipment and methods, etc. High sensitivity, short detection time, accurate and reliable results
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Embodiment 1
[0044] Embodiment 1 The implementation of the concrete technical scheme of the present invention
[0045] 1. Methods for the identification of Candida species
[0046]According to clinical reports, there are seven main types of pathogenic Candida, namely, Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis, Candida parapsilosis, Candida jiyemeng and Candida portuguese. More than 95%, therefore, this project selects seven kinds of Candida as the target strains for strain identification. According to the gene sequence of the insertion sequence ITS1 between the 18SrRNA and 5.8SrRNA of the seven Candida species, it was used as the basis for the identification of the strains. After gene sequence comparison and analysis, the insertion sequence ITS1 has certain differences in different Candida species, and it is feasible to be used as the basis for identification of Candida species.
[0047] 2. Method for detection of drug resistance of Candida albicans
[0048]...
Embodiment 2
[0063] Embodiment 2 The use of kit of the present invention
[0064] 1. Auxiliary reagents:
[0065] Liquid A: 100mL 20×SSC, 10mL 10% SDS, add pure water to 1000mL, store at room temperature.
[0066] Solution B: 25mL of 20×SSC, 10mL of 10% SDS, add pure water to 1000mL, store at room temperature.
[0067] Solution C: add 100mL of 1M sodium citrate to 1000mL with pure water, and store at room temperature.
[0068] Chromogenic solution: add 1mL TMB and 2μL 30% H to 19mL LC solution 2 o 2 .
[0069] 2. Sample processing
[0070] Method for extracting DNA from secretion samples: collect clinical vaginal secretion samples, and use a fungal DNA extraction kit to extract DNA from the samples.
[0071] 3. PCR amplification
[0072] Take out the PCR reaction solutions containing the kit of the present invention respectively, mark on the tube wall, centrifuge at 5000rpm for 2 seconds, and add 4 μL of the extracted sample DNA to be tested. Add 4 μL of positive quality control an...
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