Bacillus subtilis B51 and application of bacillus subtilis B51
A technology of Bacillus subtilis and bacterial agents, applied in the field of Bacillus subtilis B51 and its application in the prevention and treatment of Dendrobium candidum root rot, can solve serious problems, economic losses, and death in pieces
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Embodiment 1
[0039]Embodiment 1: the preparation method of Bacillus subtilis (Bacillus subtilis) B51 inoculum: inoculate Bacillus subtilis (B.subtilis) B51 in the fermentation medium, pH6.5, liquid volume in 500mL conical flask is 300mL, 28 °C-35 °C, shaker speed 120 rpm, 48 hours, the obtained fermentation liquid is the original bacterial liquid for preventing and treating Dendrobium officinale root rot.
[0040] Wherein the formula of fermentation medium is: 1000mL distilled water, beef extract 2g, peptone 5g, sodium chloride 5g, pH6.5.
Embodiment 2
[0041] Example 2: The preparation method of Bacillus subtilis (B.subtilis) B51 inoculum: Inoculate Bacillus subtilis B51 in the fermentation medium, pH 7.5, liquid volume in 500mL Erlenmeyer flask is 300mL, 28°C-35°C , shaking table speed 160 rev / min, 72 hours, the fermented liquid that obtains is the original bacterial liquid of preventing and treating Dendrobium officinale root rot.
[0042] Wherein the formula of fermentation medium is: 1000mL distilled water, beef extract 5g, peptone 10g, sodium chloride 5g, pH7.5.
Embodiment 3
[0043] Example 3: The preparation method of Bacillus subtilis (B.subtilis) B51 inoculum: Inoculate Bacillus subtilis B51 in the fermentation medium, pH 7, liquid volume in 500mL Erlenmeyer flask is 300mL, 28°C-35°C, Shaking table rotating speed 150 revs / min, 55 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium officinale root rot.
[0044] Wherein the formula of fermentation medium is: 1000mL distilled water, beef extract 3g, peptone 8g, sodium chloride 5g, pH 7.
[0045] The application of Bacillus subtilis B51 of the present invention will be described in detail below in conjunction with experimental examples.
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