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Method for tissue culture and rapid propagation of rubus coreanus

A technology of tissue culture and field soaking, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems that there are no reports on the tissue culture and rapid propagation of Rubus erythroides, and achieve the benefits of industrial production Effect

Active Publication Date: 2014-12-24
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The tissue culture and rapid propagation of many plants of the same genus have been reported, but there is no report on the tissue culture and rapid propagation of Rubus red glandularis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for culturing and rapidly propagating field bubble tissue, comprising the following steps in sequence:

[0024] (1) Disinfection and inoculation of soaked stems in fields

[0025] Take the new stems taken out in the same year and cut them into stems with axillary buds. Select the stems with a moderate degree of lignification, first use a toothbrush to gently scrub under tap water, and add an appropriate amount of detergent solution to soak for 2-4 minutes , rinsed with running water for 0.5 h, soaked the stem in 70% alcohol solution for 2 min in an ultra-clean bench, rinsed with sterile water for 3 times, immersed in 0.1% HgCl with 5 drops of Tween-80 2 Soak and disinfect in the solution for 13-14 min, then fully soak with sterile water for 3 times, inoculate in normal polarity direction into WPM basic medium supplemented with TDZ 0.5 mg / L, 6-BA 2.0 mg / L, The amount of sucrose added to the medium was 30 g / L, the amount of agar added was 7.0 g / L, the pH was 5.8...

Embodiment 2

[0044] The difference between this example and Example 1 lies in step (1): Disinfection and inoculation of the soaked stems in the field: Take the new stems of the soaked in the field that were extracted in the same year, cut them into stems with axillary buds, and divide them into mild and mild ones according to the degree of lignification. , medium and high, first use a toothbrush to gently scrub under tap water, add an appropriate amount of detergent solution to soak for 2-4 minutes, rinse with running water for 0.5 hours, and then transfer the stems to 70% alcohol solution in an ultra-clean bench Soak in water for 2 min, rinse with sterile water for 3 times, immerse in 0.1% HgCl with 5 drops of Tween-80 2 Soak and disinfect in the solution for 11-17 min, then fully soak with sterile water for 3 times, inoculate in normal polarity direction into WPM basic medium supplemented with TDZ 0.5 mg / L, 6-BA 2.0 mg / L, The amount of sucrose added to the medium was 30 g / L, the amou...

Embodiment 3

[0048] The difference between this example and Example 1 lies in step (2): Induction of clustered buds: select moderately lignified stem segments from the new stems extracted in the same year, first use a toothbrush to gently scrub under tap water, and add an appropriate amount of detergent Soak in the solution for 2-4 min, rinse with running water for 0.5 h, then transfer the stem segment to 70% alcohol solution for 2 min in an ultra-clean bench, rinse with sterile water for 3 times, immerse in 5 drops of Tween 0.1% HgCl at -80 2 Soak and disinfect in the solution for 13-14 min, then fully soak with sterile water for 3 times, inoculate in the direction of normal polarity with TDZ 0-2.0 mg / L, 6-BA 0-5.0 mg / L, IAA 0 -5.0 mg / L, CH 0-1.0 mg / L WPM basic medium, the addition amount of sucrose in this medium is 30 g / L, the addition amount of agar is 7 g / L, the pH is 5.8-6.0, culture The base was sterilized under high temperature and high pressure at 121°C for 20 min. The cultu...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of rubus coreanus. The method sequentially comprises the following steps: (1) disinfecting and inoculating a stem section of the rubus coreanus: taking a new stem, put forth in that very year, of the rubus coreanus, cutting into a moderately lignified stem section with an axillary bud, and inoculating; (2) inducing multiple shoots; (3) proliferating adventitious buds; (4) culturing roots of a regenerated plant; (5) acclimatizing and transplanting. Culture mediums with different components and proportions are adopted in the steps. With the adoption of the method provided by the invention, a rubus coreanus plant can be rapidly acquired to promote industrial production, the induction rate of the adventitious buds of the multiple shoots of the rubus coreanus is up to 92.5%, the propagation time of the adventitious buds is up to 7.2, and the transplanting survival rate of the plant is up to more than 95%.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rapid propagation of rice-cutting bubbles. Background technique [0002] Insert field bubble ( Rubus coreanus Miq.), also known as Dawupao, Wushaberry, Caizipao, Huihuilong, Two-headed grass, Two-headed busy, etc., is a small deciduous tree of the Rosaceae Rubus, distributed in Shaanxi, Gansu, Jiangsu, Anhui, Zhejiang, Jiangxi , Henan, Hubei, Sichuan and Guizhou provinces. It is used as medicine with fruits, leaves, roots, stems, and adventitious roots born on the ground. The fruit is warm in nature, sweet and sour in nature, has the effect of tonifying the kidney and consolidating essence, and is mainly used to treat impotence, seminal emission, and enuresis. , fractures, irregular menstruation and traumatic bleeding. The leaves are cool in nature, bitter and astringent in taste, and have the effects of expelling wind a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙骏威朱诚王飞娟江琼丁艳菲蔡冲
Owner CHINA JILIANG UNIV
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