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LAMP kit for Vibrio cholera in aquatic product

A Vibrio cholerae and kit technology, applied in the field of biological detection of aquatic products, can solve the problems of inaccurate determination of detection results, aerosol pollution, lack of real-time monitoring of reactions, etc., and achieve high sensitivity, strong specificity, and detection specificity strong effect

Active Publication Date: 2014-12-17
舟山出入境检验检疫局综合技术服务中心
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Problems solved by technology

However, the LAMP method used in the detection needs to add dyes after 1 hour to judge the results or use agarose gel electrophoresis for ultraviolet analysis and imaging. This has shortcomings such as aerosol pollution caused by opening the cover and lack of real-time monitoring of the reaction. make an accurate judgment

Method used

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  • LAMP kit for Vibrio cholera in aquatic product
  • LAMP kit for Vibrio cholera in aquatic product
  • LAMP kit for Vibrio cholera in aquatic product

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Embodiment Construction

[0038] The technical solutions of the present invention will be further specifically described below through specific embodiments and in conjunction with the accompanying drawings.

[0039]In the present invention, unless otherwise specified, the raw materials and equipment used can be purchased from the market or commonly used in this field. The methods in the following examples, unless otherwise specified, are conventional methods in the art.

[0040] 1. Vibrio cholerae (Vibrio cholerae VBO, purchased from Guangdong Huankai Microbial Technology Co., Ltd.).

[0041] Negative control bacteria were purchased from China Industrial Microbiology Culture Collection Center:

[0042] Vibrio parahaemolyticus (ATCC 17802), Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC6538), Vibrio vulnificus (ATCC 27562), Vibrio mimicus (CICC 10474), Vibrio alginolyticus (ATCC 17749) , Vibrio riverina (ATCC 33809).

[0043] 2. Main reagents and instruments: Nutrient Broth was purchased f...

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Abstract

The invention discloses an LAMP kit for Vibrio cholera in an aquatic product. The kit comprises a reaction buffer solution, specific primers for detecting the Vibrio cholera, a BstDNA polymerase, a Vibrio cholera DNA positive template and ultrapure water; and the specific primers for detecting the Vibrio cholera comprise a pair of inner primers FIP and BIP and a pair of outer primers F3 and B3. The kit has the characteristics of strong specificity, high sensitivity, simplicity and fastness, and can be used for real-time rapid and accurate detection of Vibrio cholera of a base.

Description

technical field [0001] The invention relates to the technical field of biological detection of aquatic products, in particular to a LAMP kit for vibrio cholerae in aquatic products. Background technique [0002] Cholera is a severe intestinal infectious disease caused by Vibrio cholera (VC). The disease has an acute onset, rapid spread, and wide coverage. It is an international quarantine infectious disease and is listed as one of the Class A infectious diseases in my country. , is one of the main foodborne pathogenic microorganisms, which always threatens and brings harm to human beings. At present, this bacterium is one of the pathogenic bacteria that must be inspected for most imported and exported aquatic products. [0003] At present, the method for detecting pathogenic microorganisms in food is mainly based on the traditional method (SN / T 1022-2010), that is, the separation and identification method. This method takes a long time, usually 5-7 days, and sometimes 10-15 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6844C12Q1/689C12Q2531/119Y02A50/30
Inventor 胡兴娟沈飚江玲丽王莲芳徐君辉邵宏宏李孝军张静周秀锦
Owner 舟山出入境检验检疫局综合技术服务中心
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