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Nanometer biosensor for synchronously detecting glucose and cholesterol

A biosensor, cholesterol technology, applied in the field of biochemical analysis

Active Publication Date: 2014-12-10
SUN YAT SEN UNIV
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Problems solved by technology

However, excess cholesterol in serum can form plaque in blood vessel channels, thereby hindering blood circulation and leading to cardiovascular disease
The methods used for cholesterol detection in the prior art mainly include fluorescence analysis, electrochemical analysis, molecular imprinting technology, etc., but the selectivity of these detection methods mostly depends on enzymes or antibodies that specifically recognize cholesterol, and are destructive and quite expensive

Method used

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  • Nanometer biosensor for synchronously detecting glucose and cholesterol

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Experimental program
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Embodiment 1

[0034] (2) Preparation of cyclodextrin-modified gold nanoparticles:

[0035] At room temperature, first take 47.0 mL of Mili-Q ultrapure water, take 10.0 mg of SH-β-CDs and 2.0 mL of 1.0% (w / w) HAuCl 4 Add it into a 100 mL round bottom flask, ultrasonically dissolve and mix evenly, then quickly add 1.0 mL of 1.0 M NaOH solution into it while vigorously stirring to make the pH reach 11, and stir in the dark for 16 hours, and the reaction quality is nearly wine red nano gold solution , purified with a dialysis bag with a molecular weight cut-off of 3500, and tested by transmission electron microscope, ultraviolet absorption spectrum (the maximum ultraviolet absorption peak is at 520 nm), particle size, and surface potential, and then used.

[0036] (3) Preparation of carbon quantum dots:

[0037] Referring to the preparation methods that have been published in the world, an aqueous solution of carbon quantum dots rich in carboxyl groups is prepared by one-step simple synthesi...

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Abstract

The invention provides a nanometer biosensor which is formed by self-assembling of carbon quantum dot-labeled concanavalin and a cholesterol sensor, wherein the cholesterol sensor is prepared by co-incubating cyclodextrin-modified nanogold and rhodamine 6G to remove excessive reactants. Moreover, the invention further provides a preparation method and application of the nanometer biosensor. The nanometer biosensor provided by the invention is established based on a fluorescence resonance energy transfer theory, can synchronously detect glucose and cholesterol, avoids mutual interference in the detection process and is a dual substance quantitative determination sensor which is novel, simple, convenient, low in cost, rapid and high in sensitivity.

Description

technical field [0001] The invention belongs to the field of biochemical analysis, and more specifically relates to a nano biosensor for synchronous detection of glucose and cholesterol, a preparation method and application thereof. Background technique [0002] FRET, that is, fluorescence resonance energy transfer, is a technology developed earlier. With the development of green fluorescent protein application technology, FRET has become a powerful tool for detecting the nanoscale distance and nanoscale distance changes of biological macromolecules in the living body. It has a wide range of applications in molecular interaction analysis, cell physiology research, and immune analysis. Fluorescence resonance energy transfer is an energy transfer phenomenon between two fluorescent molecules that are very close together. When the emission spectrum of the donor fluorescent molecule overlaps with the absorption spectrum of the acceptor fluorescent molecule, and the distance betw...

Claims

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Application Information

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IPC IPC(8): G01N33/92
CPCG01N33/532G01N33/66G01N33/92
Inventor 易长青张肇敏陈俊英张璐璐时宇鹏潘益张恒
Owner SUN YAT SEN UNIV
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