Staphylococcus aureus detection method

Abstract

The invention discloses a staphylococcus aureus detection method. The method comprises the following steps: preparing staphylococcus aureus culture solution I; adding a sample to be detected in the culture solution I for enrichment culture to prepare culture solution II for standby use; preparing an optical fiber biosensor: enabling protein specific antibody I of staphylococcus aureus A to be immobilized on the optical fiber biosensor under the hydrophobic forces and the intermolecular forces or under the action of covalent bonds; preparing blank detection liquid and liquid to be detected, wherein the blank detection liquid comprises the culture solution I and buffer solution, the liquid to be detected comprises the culture solution II and buffer solution; immersing the prepared optical fiber biosensor into the blank detection liquid for equalization treatment, and then immersing into the liquid to be detected for detection. Compared with the detection method in the prior art, the staphylococcus aureus detection method has the advantages of real-time detection, short detection time, convenience in operation and the like and has good application prospect.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to a detection method for Staphylococcus aureus. Background technique [0002] Staphylococcus aureus, belonging to the genus Staphylococcus, is a common Gram-positive bacterium that is widely distributed in nature and exists in air, water, soil, dust, and human and animal excrement , is an opportunistic pathogen. Because Staphylococcus aureus widely exists in the surrounding environment, food is easily contaminated by Staphylococcus aureus, and eating food contaminated by Staphylococcus aureus can cause skin and soft tissue infections, sepsis, mastitis, endocardial infection in humans and animals Inflammation, pneumonia, enteritis, meningitis, osteomyelitis, fasciitis, arthritis, toxic shock syndrome, etc. Poisoning caused by Staphylococcus aureus is most common in developed countries such as North America and Europe. According to relevant statistics, the number of f...

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Problems solved by technology

[0004] The traditional detection method of Staphylococcus aureus mostly adopts the combination of isolation culture, biochemical test and plasma coagulation enzyme test. This method is not only time-consuming, cumbersome to operate, high in cost, but also requires experienced testers.

With the continuous development of biotechnology, molecular biology detection methods are widely used in the detection of microorganisms, but molecular biology detection also relies on professional and technical personnel, special environmental facilities, expensive equipment

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