Apolipoprotein E detection kit

Abstract

The invention discloses an apolipoprotein E detection kit which comprises a reagent R1, a reagent R2 and a calibration product. The reagent R1 mainly comprises the following components: 10-50mmol / L of phosphate buffer, 10-40g / L of polyethylene glycol, 50-200mmol / L of sodium chloride, 0.02-0.2% of tween 20, 0.1-1% of BSA (Bovine Serum Albumin) and 0.1% of NaN3, wherein polyethylene glycol is low-polymerization polyethylene glycol. The reagent R2 mainly comprises the following components: 10-50mmol / L of phosphate buffer, 50-200mmol / L of sodium chloride, 0.02-0.2% of tween 20, 10-50% (v / v) of ApoE antibody and 0.1% of NaN3; the calibration product mainly comprises the following components: 10-50mmol / L of phosphate buffer, 50-200mmol / L of sodium chloride, 0.1-1% of BSA, 0.2-5mmol / L of EDTA (Ethylene Diamine Tetraacetic Acid), 0.02-0.2% of tween 20, 0.1% of NaN3 and 0-120mg / L of ApoE antigen. The apolipoprotein E detection kit is convenient to use, good in reagent stability, good in detection result accuracy, good in repeatability, convenient to use, low in cost and convenient to popularize and apply.

Description

[0001] technical field [0002] The invention relates to a human biological protein detection technology, in particular to an apolipoprotein E detection kit. [0003] The meanings of the following expressions in this application are: [0004] 0.2M sodium dihydrogen phosphate: indicates that the concentration of sodium dihydrogen phosphate in the solution is 0.2mol / L; [0005] 0.1% NaN 3 : Indicates adding NaN to every 100mL solution 3 0.1g; [0006] ApoE antibody 10-50% (v / v): the amount of ApoE antibody added in reagent R2 accounts for 10-50% of the total volume of reagent R2, in ml / ml; [0007] BSA: bovine serum albumin; [0008] EDTA: ethylenediaminetetraacetic acid; [0009] PBS: phosphate; [0010] The meanings expressed in this application that are similar to the above expressions are similar to the above meanings; unless otherwise stated in the application. [0011] Background technique [0012] Apolipoprotein E (ApoE) is a glycoprotein of 299 amino acids bou...

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Problems solved by technology

[0013] Known methods for measuring ApoE include chromatography, electrophoresis, and immunoassay. Among them, chromatography and electrophoresis are cumbersome to operate, and they cannot perform batch sample analysis and directly go to full-automatic biochemical analyzers. Immunodiffusion, radioimmunoassay, fluorescence-labeled immunoassay, enzyme-labeled immunoassay, and chemiluminescence immunoassay also have many deficiencies; if special equipment is required, the sample needs to be processed, and it cannot be used on a fully automatic biochemical analyzer Perform batch monitoring and analysis, etc.

The immunoturbidimetric method commonly used in clinical practice is popular because of its small amount of sample, which can be directly analyzed in batches on a fully automatic biochemical analyzer, and is easy to operate; however, the currently established methods and reagents have some shortcomings and deficiencies, mainly in : First, the antibody titer is not high, and the specificity, affinity, and affinity are not good; second, the calibration serum is based on human serum, although the human serum with negative hepatitis B surface antigen, negative HIV test, and normal alanine aminotransferase However, it is also difficult to rule out the possibility that there are no other infectious diseases, which brings little danger to the operator; and the calibration serum is a freeze-dried product, and the value of each batch is different. It needs to be reconstituted with water before use. It is extremely inconvenient; in addition, after reconstitution, it must be frozen and stored, especially it cannot be thawed repeatedly, which not only causes waste of reagents, but also cannot guarantee the quality, the measurement results vary greatly, and its stability is not good; the third is for high-fat, The anti-interference ability of jaundice and hemolysis samples is poor; the fourth is that the single-point calibration solution is used for calibration, which does not conform to the calculation of the curve regression equation, resulting in the phenomenon of low high values ​​and high low values, and the measured results are inaccurate; fifth, antiserum Precipitation occurs in a short period of time, making it difficult to operate on the machine and affecting the detection effect

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