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A troponin detection kit with good precision and strong linear correlation

A linear correlation and detection kit technology, which is applied in the field of troponin detection kits, can solve the problems of changing the light transmission performance of the reaction solution and low sensitivity, and achieve the effect of high sensitivity and good correlation

Active Publication Date: 2016-05-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The principle is that when the microspheres cross-linked with antibodies react with the antigen to agglutinate, the light transmission properties of the reaction solution are changed, but there is also the problem of low sensitivity.

Method used

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  • A troponin detection kit with good precision and strong linear correlation
  • A troponin detection kit with good precision and strong linear correlation
  • A troponin detection kit with good precision and strong linear correlation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Reagent R1:

[0040] PIPES (piperazine-N,N-bis(2-ethanesulfonic acid)) buffer

[0041] Polyethylene glycol 200mmol / L

[0042] Sodium azide 8mmol / L

[0043] Sodium edetate reagent 4mmol / L

[0044] Ethanolamine 2mmol / L;

[0045] Reagent R2:

[0046] PIPES buffer

[0047] Microsphere particles bound with troponin monoclonal antibody 200mmol / L

[0048] Tween - 205%

[0049] Sodium azide 3mmol /

[0050] Sodium edetate 2mmol / L

[0051] Bovine serum albumin 3mmol / L.

[0052] Measure the change of absorbance under the condition of 500nm of automatic biochemical analyzer:

[0053] Automatic biochemical analyzer parameter settings:

[0054] (a) Detection temperature: 37°C

[0055] (b) Detection wavelength: the main wavelength is 500nm; the secondary wavelength is set to 0;

[0056] (c) Reaction time: 10 minutes. Among them, the incubation time is 3 minutes, the reaction time is 100 seconds, and the detection time is 200 seconds.

[0057] Detection steps

[0058] S...

Embodiment 2

[0076] Reagent R1:

[0077] PIPES (piperazine-N,N-bis(2-ethanesulfonic acid)) buffer

[0078] Polyethylene glycol 220mmol / L

[0079] Sodium azide 10mmol / L

[0080] Sodium edetate reagent 5mmol / L

[0081] Ethanolamine 2mmol / L

[0082] Reagent R2:

[0083] PIPES buffer

[0084] Microsphere particles bound with troponin monoclonal antibody 220mmol / L

[0085] Tween - 205%

[0086] Sodium azide 5mmol /

[0087] Sodium edetate 3mmol / L

[0088] Bovine serum albumin 5mmol / L.

[0089] The volume ratio of R1 and R2 is 3:1; the determination method is the same as above.

Embodiment 3

[0091] Reagent R1:

[0092] PIPES (piperazine-N,N-bis(2-ethanesulfonic acid)) buffer

[0093] Polyethylene glycol 200mmol / L

[0094] Sodium azide 5mmol / L

[0095] Sodium edetate reagent 1mmol / L

[0096] Ethanolamine 2mmol / L

[0097] Reagent R2:

[0098] PIPES buffer

[0099] Microsphere particles bound with troponin monoclonal antibody 200mmol / L

[0100] Tween - 205%

[0101] Sodium azide 1mmol /

[0102] Sodium edetate 0.5mmol / L

[0103] Bovine serum albumin 1mmol / L.

[0104] The volume ratio of R1 and R2 is 3:1; the determination method is the same as above.

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Abstract

The invention relates to a tropnin detection kit with good precision and high linear correlation. The troponin detection kit comprises a reagent R1 and a reagent R2 which are in a volume ratio of 3 to 1, wherein the reagent R1 consists of PIPES (piperazine-N, N-bis(2-ethanesulfonic acid)) buffer solution, polyethylene glycol, sodium azide, sodium ethylene diamine tetracetate reagent and neovaricaine; the reagent R2 consists of PIPES buffer solution, microsphere particles which are combined with troponin monoclonal antibody, tween-20, sodium azide, sodium ethylene diamine tetracetate and bovine serum albumin. The sensitivity and the linear correlation of the detection kit are better than those of the existing detection kit.

Description

technical field [0001] The invention relates to a troponin detection kit with good precision and strong linear correlation, which belongs to the technical field of in vitro diagnosis. Background technique [0002] Troponins are structural proteins involved in the regulation of striated muscle and cardiac contractility. Troponin is composed of three subunits: troponin T (TnT), troponin I (TnI) and troponin C (TnC). Among them, TnI is the inhibitory subunit of myofibrillar AFPase, with a molecular weight of 18kD. Troponin I wraps around the central helix of TnC and becomes an integral part of the Ca2+ binding site. The combination of the three subunits forms a complex with tropomyosin to regulate the contractility and speed of muscle cells. Cardiac troponin I (CTnI) is uniquely distinct from troponin I found in skeletal muscle. Cardiac troponin I has an additional 31 amino acid sequence at the N-terminus that makes CTnI specific for the myocardium and has been extensively ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/68G01N21/31
CPCG01N33/54313G01N33/577G01N33/6887
Inventor 李静甘宜梧王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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