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Method for obtaining hedychium coronarium tetraploid plants by slice culture technology

A white ginger flower and tetraploid technology is applied in the field of obtaining a white ginger flower tetraploid plant by using a thin slice culture technology, which can solve the problems of successful induction of polyploid plants without the white ginger flower thin plate culture technology, and achieves easy popularization and reliability. The effect of promotion, strong operability, and simplified screening process

Active Publication Date: 2014-12-03
GUANGDONG UNIV OF EDUCATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The currently reported plant regeneration systems of Gingeria in vitro mainly use hypocotyls of aseptic seedlings with mature seeds as explants, or use adventitious buds to induce cluster buds to reproduce and regenerate complete plants. and reports of successful induction of polyploid plants

Method used

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  • Method for obtaining hedychium coronarium tetraploid plants by slice culture technology
  • Method for obtaining hedychium coronarium tetraploid plants by slice culture technology
  • Method for obtaining hedychium coronarium tetraploid plants by slice culture technology

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Experimental program
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Effect test

Embodiment 1

[0063] (1) Disinfection of explants and induction of adventitious buds: Get the new shoots germinated from the rhizomes of Hedychium coronarium (commercially available routine. The same below), rinse them with tap water and soak them in detergent for 30 minutes. Minutes, then rinsed with tap water for 30 minutes, then sterilized the surface with 70wt% alcohol for 60 seconds, and finally sterilized with 0.1wt% mercury chloride for 18 minutes, cut 0.5cm buds on the ultra-clean workbench as explants, and inoculated the buds Cultivate in induction medium A, and induce adventitious buds after 20 days of cultivation; the described bud induction medium A is MS medium + 3mg L -1 6-BA+0.2mg·L -1 NAA+30g·L -1 Sucrose+7g·L -1 Agar;

[0064] (2) Adventitious buds become seedlings: the adventitious buds induced by step (1) are transferred to the seedling medium to promote the rooting and maturation of the seedlings, and after 15 days of cultivation, strong test-tube plantlets with a hei...

Embodiment 2

[0081] (1) Disinfection of explants and induction of adventitious buds: Take the new shoots germinated from the rhizomes of Hedychium coronarium, rinse them with tap water, soak them in detergent for 28 minutes, and then rinse them with tap water for 28 minutes. Minutes later, carry out surface disinfection with 75wt% alcohol for 75 seconds, and finally sterilize with 0.1wt% mercuric chloride for 15 minutes, cut 1.0cm buds on the ultra-clean workbench as explants, inoculate them into bud induction medium A and cultivate them. After 30 days of cultivation, adventitious buds can be induced; the bud induction medium A is MS medium+4mg L -1 6-BA+0.1mg·L -1 NAA+30g·L -1 Sucrose+7g·L -1 Agar;

[0082] (2) Adventitious buds become seedlings: the adventitious buds induced by step (1) are transferred to the seedling medium to promote the rooting and maturation of the seedlings, and cultivate for 30 days to obtain strong test-tube plantlets with a height of more than 6 cm; described ...

Embodiment 3

[0092] (1) Disinfection of explants and induction of adventitious buds: Take the new shoots germinated from the rhizomes of Hedychium coronarium, wash them with tap water, soak them in detergent for 25 minutes, and then rinse them with tap water for 25 minutes. Minutes later, carry out surface disinfection with 72wt% alcohol for 90 seconds, and finally disinfect with 0.1wt% mercuric chloride for 20 minutes, cut 1.5cm buds on the ultra-clean workbench as explants, inoculate them into bud induction medium A and cultivate them. Adventitious buds were induced after 40 days of cultivation; the bud induction medium A was MS medium+5mg L -1 6-BA+0.15mg·L -1 NAA+30g·L -1 Sucrose+7g·L -1 Agar;

[0093] (2) Adventitious buds become seedlings: the adventitious buds induced by step (1) are transferred to the seedling medium to promote the rooting and maturation of the seedlings, and they can be cultivated for 45 days to obtain strong test-tube plantlets with a height of more than 6 cm;...

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Abstract

The invention discloses a method for obtaining hedychium coronarium tetraploid plants by a slice culture technology. The method comprises the following steps: cutting a slice from a tube seedling stem section, processing the slice by using colchicines, and then carrying out adventitious bud induction on the processed slice by adopting the slice culture technology to obtain regenerated plants; detecting the regenerated plants; and screening to obtain the tetraploid plants. According to the method, when induced mutation is carried out, the used explants are the slices, only are 0.3-0.5mm in thickness, and are few in cells; when treatment is carried out by using colchicine, the cells of the explants can be fully exposed in a liquid medicine; most of the obtained plants are homozygote; the screening processes of polyploid plants are simplified; and the used chromosome number analysis technology has the advantages of being simple in operation, short in required time, clear in obtained chromosome image and the like. The method disclosed by the invention has the advantages of being simple in operation, high in plant regeneration frequency, short in regeneration cycle, easily available in materials, strong in operability and easy to popularize and promote.

Description

technical field [0001] The invention belongs to the technical field of Hedychium coronarium breeding in Zingiberaceae, and particularly relates to a method for obtaining tetraploid plants of Hedychium coronarium by means of slice culture technology. The rapid propagation culture system of white ginger flower was established by thin slice culture technology, and by using the established rapid propagation culture system, combined with colchicine treatment, polyploid plants of white ginger flower could be obtained quickly, so as to realize the improvement of white ginger flower variety, The purpose of acquiring new species. Background technique [0002] White ginger flower is a perennial rhizome herbaceous flower plant belonging to the genus Zingiberaceae. Because of its bright colors and fragrant smell, it is mainly used as fragrant fresh cut flowers, garden greening and garden landscape plants. It is a popular garden aromatic flower. The market demand is huge. In addition, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 肖望涂红艳袁学文邓崇会黄少丽张琳伟张棣升
Owner GUANGDONG UNIV OF EDUCATION
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