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Rapid propagation method for rosemary suspension cell culture

A technology of suspended cells and rosemary, which is applied in the field of plants, can solve the problems of weak regeneration ability and slow growth of rosemary, and achieve the effects of short growth cycle, large-scale production and low energy consumption

Inactive Publication Date: 2014-11-26
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rosemary likes a warm climate, but it grows slowly during the high temperature period on the plains of Taiwan. The temperature without cold currents in winter is more suitable for its growth. In terms of water supply, rosemary leaves are leathery and more drought-tolerant. Therefore, the soil for planting is Rich in sand and good drainage is good for growth and development, it is worth noting that rosemary grows slowly, which also means that it is not very regenerative
At present, the main propagation methods are seed propagation and cutting propagation. The tissue culture technology is easy to operate and has strong regeneration ability. It is still to be studied.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Take rosemary seeds, soak them in bleaching powder for 3 minutes, remove the hair on the surface with a brush, wash them with running water for 2 hours, disinfect them with sodium hypochlorite on the ultra-clean workbench for 17 minutes, rinse them with sterile water 5 times, absorb the surface moisture with absorbent paper, insert 1 / In 2MS medium, obtain aseptic seedlings, and use the 0.2-0.5cm long tender stems in the upper part of the aseptic seedlings as explants, inoculate into MS+2,4-D0.5mg / L+KT0.3mg / L Carry out callus induction in the culture medium, add 7g / L agar, 30g / L sucrose, low light 1000-1500lx, dark room induction, the rosemary callus that comes out is put into the liquid medium without adding agar, add 1.0mg / L 2,4-dichlorophenoxyacetic acid for suspension cell culture, add galactose 50g / L, agar 7g / L, pH6.0, light 3000lx, temperature 24°C, cultured suspension cells were transferred to solid culture The callus after basal proliferation is screened. The te...

Embodiment 2

[0012] Take rosemary seeds, soak them in bleaching powder for 3 minutes, remove the hair on the surface with a brush, wash them with running water for 2 hours, disinfect them with sodium hypochlorite on the ultra-clean workbench for 17 minutes, rinse them with sterile water 5 times, absorb the surface moisture with absorbent paper, insert 1 / In 2MS medium, obtain aseptic seedlings, and use the 0.2-0.5cm long tender stems in the upper part of the aseptic seedlings as explants, inoculate into MS+2,4-D0.5mg / L+KT0.3mg / L Carry out callus induction in the culture medium, add 7g / L agar, 30g / L sucrose, low light 1000-1500lx, dark room induction, the rosemary callus that comes out is put into the liquid medium without adding agar, add Suspension cell culture with 5.0mg / L 2,4-dichlorophenoxyacetic acid, galactose 50g / L, agar 7g / L, pH6.0, light 3000lx, temperature 24°C, cultured suspension cells were transferred to solid culture The callus after basal proliferation was screened and the g...

Embodiment 3

[0014] Take rosemary seeds, soak them in bleaching powder for 3 minutes, remove the hair on the surface with a brush, wash them with running water for 2 hours, disinfect them with sodium hypochlorite on the ultra-clean workbench for 17 minutes, rinse them with sterile water 5 times, absorb the surface moisture with absorbent paper, insert 1 / In 2MS medium, obtain aseptic seedlings, and use the 0.2-0.5cm long tender stems in the upper part of the aseptic seedlings as explants, inoculate into MS+2,4-D0.5mg / L+KT0.3mg / L Carry out callus induction in the culture medium, add 7g / L agar, 30g / L sucrose, low light 1000-1500lx, dark room induction, the rosemary callus that comes out is put into the liquid medium without adding agar, add Suspension cell culture with 3.0 mg / L 2,4-dichlorophenoxyacetic acid, galactose 50g / L, agar 7g / L, pH 6.0, light 3000lx, temperature 24°C, cultured suspension cells were transferred to solid culture The callus after basal proliferation is screened. The ten...

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Abstract

The invention researches a rapid propagation method for rosemary suspension cell culture. The rapid propagation method comprises the steps of obtaining germfree seedlings, inducing calluses, culturing and optimizing suspension cells, regenerating plants and the like. Rosemary suspension cells prepared by the rapid propagation method are high in proliferation rate, short in growth period and high in secondary metabolite, and are favorable for development and utilization of medicinal components of rosemary.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of rosemary, belonging to the technical field of plants. Background technique [0002] Rosemary, Lamiaceae, Rosmarinus officinalis , another name: Ocean Dew, shrub, distribution area: China, the Mediterranean, Europe, the United States, native to Europe and the Mediterranean coast of North Africa, it was introduced to China during the Cao Wei Dynasty, and occasionally introduced and cultivated in Chinese gardens. The leaves have a tea aroma, pungent and slightly bitter taste, and are often used in cooking, and can also be used to make herbal tea. An evergreen shrub, rosemary was believed to enhance memory in ancient times, and it is currently recognized as the most antioxidant plant. The antioxidant components in rosemary are mainly carnosic acid, carnosol, rosmanol, ursolic acid, rosmarinic acid and other components, which can eliminate flatulence, enhance memory, refresh the min...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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