A detection method for microcystin content in aquatic products
A microcystin and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to distinguish specific homologues, low sensitivity and specificity, and heavy production workload, and achieve The detection method is accurate and convenient, the selectivity is good, and the test is fast and safe.
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[0027] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other.
[0028] Such as figure 1 As shown, the implementation steps of this embodiment are mainly divided into four steps of configuring microcystin standard solution, drawing a standard curve, sample pretreatment and carrying out chromatographic analysis to the test solution and calculating the concentration of microcystin in the sample. The specific operation is:
[0029] A. Prepare microcystin standard solution: Dilute the microcystin standard solution with methanol to prepare MC-LR and MC-RR standard solutions with concentrations of 0.30, 0.50, 1.00, 5.00 and 10.00 μg / ml in sequence.
[0030] B. Draw a standard curve: perform high-performance liquid chromatography analysis on multiple microcystin standard solutions with different concentrations of 0.30, 0.50, 1.00, 5.00 and 10.00 μg / ml in sequence as describe...
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