Method for detecting content of microcystic toxins in aquatic product

Abstract

The invention discloses a method for detecting the content of microcystic toxins in an aquatic product. The method is characterized by comprising the following steps of (1) respectively preparing a plurality of standard working solutions of the microcystic toxins with gradient concentrations, respectively performing chromatographic analysis on the standard working solutions to obtain peak areas, and drawing a standard curve by respectively taking the gradient concentrations and the peak areas as transverse and longitudinal coordinates; and (2) sucking a set volume of a sample solution for chromatographic analysis, measuring the peak area of each sample solution, and calculating the concentration of the microcystic toxins in the sample solution according to the corresponding standard curve in the step (1). The detection method disclosed by the invention is accurate and convenient, and the relative standard deflection is less than 2 percent; the detection limit is 0.01 microgram per kilogram; furthermore, an organic solvent is not required, and the testing is quick and safe; the detection method is high in selectivity and high in sensitivity, integrates sampling, extraction, concentration and sample injection, and can be used with a liquid chromatograph; the manual operation and the automatic operation can be realized; and therefore, a sample treatment technology and the analysis operation are simple, and time is saved; the detection method is very suitable for research on the content of the microcystic toxins in the aquatic product.

Description

technical field [0001] The invention relates to a method for detecting toxin content, in particular to a method for detecting microcystin in aquatic products. Background technique [0002] Microcystins (microcystins, MCs) are a class of natural hepatotoxins with monocyclic heptapeptide structure produced by cyanobacteria, and are the most common blue-green algae toxins in water. MC-LR is the most prevalent, relatively abundant, and highly toxic MC known to exist. Others such as MC-YR, MC-RR, MC-LF, and MC-LW are also common. MC is very stable under sunlight, but when exposed to ultraviolet rays, it can be hydrolyzed or undergo chemical isomerization and chemical bonding reactions to lose toxicity, especially when the ultraviolet wavelength is close to the maximum absorption wavelength of its absorption peak, MC is rapidly degraded. Algae toxins have high heat resistance, and even heating and boiling can neither destroy the toxins nor remove them. [0003] MC widely exists...

AI Technical Summary

Problems solved by technology

The biological test method is simple and quick to operate, and the result is intuitive, but it consumes more toxins, and the sensitivity and specificity are not high. The minimum research line should be measured by half-lethal dose and lethal dose; the enzyme-linked analysis method is simple to operate and has high sensitivity. , can detect different homologues of the toxin, but recognition of multiple homologues requires broad-spectrum antibodies; protein phosphatase inhibition assays have the disadvantage of not being able to distinguish specific homologues, and cytotoxin research techniques are production workloads Large, the minimum detection limit is 10~20mg / ml, the accuracy is not high

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